优化TTC-脱氢酶还原法测定陶粒负载微生物活性  

Study on determination of biofilm activity in BAF by TTC-dehydrogenase assay

作　　者：齐鲁青[1] 汪晓军[1] 詹德明[1] 

机构地区：[1]华南理工大学环境科学与工程学院,工业聚集区污染控制与生态修复教育部重点实验室,广东广州51000

出　　处：《现代化工》2012年第3期93-96,共4页Modern Chemical Industry

基　　金：国家自然科学基金项目(51078149);广州市科技计划资助项目,(2009Z1-E751)

摘　　要：利用氯化三苯基四氮唑(TTC)-脱氢酶还原法定量测定了曝气生物滤池中陶粒负载生物膜的活性,并对影响生物活性测定的因素进行了分析和优化。实验表明,经优化后得出最佳培养条件是:陶粒采用原位法制样,依次加入pH为8.0的Tris-HCl缓冲液,质量分数为0.4%的TTC溶液,0.1 mol/L葡萄糖溶液,在温度40℃下培养4 h,最后生成的三苯基甲臢(Triphenyl Formazone,TF)用甲苯萃取可以除去有色废水的颜色影响。采用TTC-脱氢酶还原法可以快速、方便的检测曝气生物滤池中陶粒负载生物膜的生物活性,并能很好的进行定量化测定。The biofilms activity in Biological Aerated Filters （BAF） is quantitatively determined by 2,3,5- triphenyl tetrazolium chloride （TTC）-dehydrogenase assay and some major factors influencing the determination are studied in this paper. The optimum parameters are shown as follows ： Tris-HCl buffers and 8.0 of pH, O. 4% （ mass fraction） of Trc and 0. 1 mol/L of dextrose solution. The incubation time and temperature are 4 h and 40℃, respectively. The formazan reduction product can be efficiently extracted by methylbenzene, which can reduce the disturbance of dye color. This study demonstrates that the TTC-dehydrogenase assay can be successfully applied to the determination of biofilms activity in BAF.

关 键 词：TTC-脱氢酶还原法 曝气生物滤池 生物活性 优化 

分 类 号：S567[农业科学—中草药栽培]