人巨细胞病毒UL147基因在婴儿巨细胞病毒肝炎临床株中的多态性  

Sequence polymorphisms of the UL147 gene in clinical strains of human cytomegalovirus

作　　者：尹晓波[1] 毛志芹[1] 常淑婷[1] 吉耀华[2] 何蓉[2] 齐莹[2] 阮强[2] 孙梅[1] 马艳萍[2] 孙峥嵘[2] 

机构地区：[1]中国医科大学附属盛京医院小儿消化内科,辽宁省沈阳市110004 [2]中国医科大学附属盛京医院病毒室,辽宁省沈阳市110004

出　　处：《世界华人消化杂志》2012年第4期282-288,共7页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30700916~~

摘　　要：目的:探讨人巨细胞病毒(human cytomegalov-irus,HCMV)UL147基因在婴儿巨细胞病毒肝炎临床株中的多态性及其与致病性之间的关系.方法:对25株经荧光定量PCR方法检测HCMVDNA为阳性的临床株的尿液上清液进行HCMVUL147全序列PCR扩增,并对PCR扩增产物进行序列测定及分析.结果:25株临床株扩增阳性产物进行HCMVUL147全序列PCR扩增,结果有19株PCR扩增阳性;与HCMVToled株进行序列比较分析,所有UL147开放读码框架(open-reading-fraem,ORF)长度在477-480bp,序列呈现较高多态性,UL147基因变异率为2.7%-10.9%;氨基酸变异集中在30-40氨基酸位点,变异率为4.3%-8.75%;所有研究标本中没有序列与Toledo完全一致.序列差异多位于序列的5'端,巨细胞病毒肝炎患儿和无症状患儿之间的UL147基因DNA序列是一致的.NCBIPROS数据库预测UL147编码蛋白功能区显示几乎所有的UL147序列中都存在1个CKP和1个PKC位点.结论:巨细胞病毒肝炎患儿临床株中的HCMVUL147基因序列呈现高度多态性.UL147基因多态性与其对肝脏损害的程度无关.AIM：To investigate the sequence polymorphisms in the open reading frame（ORF） of the UL147 gene in clinical strains of cytomegalovirus and to identify the relationship between UL147 sequence polymorphism and cytomegalovirus hepatitis.METHODS：The ORF of the UL147 gene in 19cytomegalovirus strains isolated from infants with cytomegalovirus hepatitis was amplified by PCR and sequenced.RESULTS：In comparison with the sequence of the Toledo strain,strain-specific polymorphisms were present in the 5＇ end of the UL147 ORF,and the polymorphisms were located in the 5＇ end of sequence.The UL147 ORF varied from 477-480 kb.No sequence differences were found between strains isolated from infants with cytomegalovirus hepatitis and asymptomatic patients.CONCLUSION：The ORF of the UL147 gene is hypervariable in different cytomegalovirus strains.The conservation of predicted UL147 product in clinical strains suggests that UL147 gene polymorphisms have no relationship with the severity of cytomegalovirus hepatitis.

关 键 词：人类巨细胞病毒 UL147基因 多态性 定量PCR 

分 类 号：R725.7[医药卫生—儿科]