茵陈对妊娠期肝内胆汁淤积症模型大鼠血清及胎盘组织内皮素的影响  

Effect of Herba artemisiae scopariae decoction on serum and placental tissue levels of pregnancy

作　　者：顾江红[1] 周玲贞[1] 徐道芬[1] 贡方红[1] 朱珍[1] 杜兰芳[1] 宁维翾[1] 

机构地区：[1]杭州市中医院妇产科,310007

出　　处：《浙江医学》2012年第4期281-283,共3页Zhejiang Medical Journal

基　　金：杭州市卫生局重点项目（编号2009Z07）

摘　　要：目的研究茵陈水煎剂对妊娠期肝内胆汁淤积症（ICP）模型大鼠血清及胎盘组织内皮素（ET）的影响。方法以苯甲酸雌二醇针剂复制SD大鼠ICP模型；以50％茵陈水煎剂灌胃；采用ABC—ELISA法测定大鼠血清及胎盘组织ET值。结果茵陈治疗可增加胎鼠平均体重，降低胎鼠死亡率，降低孕鼠血清及胎盘组织ET水平。结论茵陈能显著降低ICP大鼠血清及胎盘组织ET水平，明显改善妊娠结局。Objective To investigate the effects of Herba artemisiae scopariae on endothelin （ET） levels in rats with intrahepatic cholestasis of pregnancy （ICP）, Methods Sixty 15-day pregnant SD rats were randomly divided into three groups： control group, ICP group and treatment group. ICP was induced by subcutaneous injection with estradiol benzoate （5mgokg-lod-1） and subsequent intragastric administration of 5mlod-1 normal saline for 5 d; in treatment group ICP rats received intragastric administration of 50% Herba artemisiae scopariae water decoction; and in the control group, rats received subcutaneous injection of 5ml normal saline plus intragastric perfusion with 5mlod-1 normal saline for 5 d. The serum and placental tissue levels of ET were determinate by ELISA-ABC method. Results The ET levels in serum and placental tissue were significantly higher in ICP group than those in control group （P〈0.01）. The body weight and fetal survival rate in ICP group were decreased significantly as compared with those in control group （P〈0.01）. The average weight was higher and fetal mortality rate was lower in treatment group than those in ICP group （both P〈0.05 ）; while the serum and placental tissue ET levels in treatment group were decreased compared with ICP group（P〈0.05）. Conclusion The increased endothelin levels in plasma and placental tissue in ICP rats may be associated with fetal complications and Herba artemisiae scopariae can significantly reduce ET levels and improve the outcome of pregnancy in rats.

关 键 词：妊娠期肝内胆汁淤积症 茵陈 内皮素 胎盘 

分 类 号：R714.255[医药卫生—妇产科学]