炒黄川楝子乙醇提取物对大鼠血液生化学及血液细胞学的影响  

Study on the Effect of Ethanol Extraction of Melia toosendan on Blood Biochemistry and Cytology of Rats

作　　者：向晓雪[1] 唐大轩[2] 熊静悦[2] 梁娅君[1] 牟道华[1] 杨小卫[1] 韩敏[1] 谭正怀[2] 

机构地区：[1]成都中医药大学,四川成都610072 [2]四川省中医药科学院,四川成都610041

出　　处：《四川中医》2012年第3期62-65,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家"973"计划项目"确有疗效的有毒中药科学应用关键问题的基础研究--有毒中药毒效学和靶器官毒作用规律研究"(2009CB522801)

摘　　要：目的:观察长期给予炒黄川楝子乙醇提取物(CLZ)对大鼠血液生化及血液细胞的影响。方法:将5～6周龄,雌雄各半的SD大鼠80只随机分为四组,分别灌胃CLZ大剂量组(6g/kg),CLZ中剂量组(3g/kg),CLZ小剂量组(1.5g/kg)以及等体积的蒸馏水,每天给药,连续给药30天,部分动物停药后恢复2周。在末次给药后24小时并在禁食不禁水16小时后,取血测定其生化学指标以及血液细胞学指标。结果:血液生化检测结果显示:药物组AST、ALT、BUN显著升高,TP、GLU值减少;血液细胞学研究结果显示:药物组MCV、MCH、网织红细胞减少,RBC值升高。结论:连续给药CLZ后,可呈剂量依赖性对肝脏、肾脏以及造血系统产生毒性作用,但其毒性作用是可以恢复的,其作用机理还有待进一步探讨。Objective： To evaluate the long-term toxicity of Melia toosendan to rats.Methods： 5～6weeks old,80 SD rats（Male and female evenly） were divided randomly into four groups.T he rats were respectively administered CLZ6,3 and 1.5g/kg or water,once a day for 30 days.On 31th and 45th day the rats were killed to measured the blood biochemistry and cytology.Results： Melia toosendan significantly increased the AST,ALT,BUN,RBC and decreased the levels of TP,GLU in serum,and decreased the levels of MCV,MCH and reticulocyte in blood of rats.Conclusion： Taking CLZ for long term may produce toxic effects on the kidney,liver and hematopoietic system in rats,but they are restorability.

关 键 词：川楝子 大鼠 长期毒性试验 血液生化 血液细胞学 

分 类 号：R249[医药卫生—中医临床基础]