高效液相色谱法测定马钱子散中士的宁的含量  

HPLC Determination of Strychnine in Nux Vomica Powder

作　　者：徐涛 张颖[2] 于佳 

机构地区：[1]黑龙江省食品药品监督管理干部学校 [2]黑龙江中医药大学中医药研究院 [3]黑龙江双鹤医药有限责任公司,黑龙江哈尔滨150040

出　　处：《黑龙江医药》2012年第1期11-12,共2页Heilongjiang Medicine journal

摘　　要：目的:建立马钱子散中士的宁含量的高效液相色谱测定方法。方法:采用Diamonsil-C18柱(250mm×4.6mm,5μm),以甲醇-含0.1%甲酸和0.1%二乙胺的水溶液为流动相,流速为1.0mL·min-1,检测波长为254nm。结果:士的宁进样量在0.0073～0.0584mg·mL-1浓度呈良好的线性关系(r=0.9996),平均回收率为97.94%,RSD=1.0%(n=6)。结论:该方法简便、准确、快速,适用于马钱子散中士的宁的含量测定。：Objective：To develop a reversed phase HPLC method for determining the content of strychnine in Nux Vom- lea powder. Methods：The HPLC separation was performed on a Diamonsil Cl8 column （250 mm x4. 6 ram,5 Ixm）. The mo- bile phase was a mixture of methanol and water containing 0. 1% formic aeid and 0. 1% diethylamine and delivered at a flow rate of 1.0 mg · mL-1. The detection was done at 254 nm. Results：The ealibration curve was found to be linear in the con eentration range of 0. 0073 ~ 0. 0584 mg · mL-l （ r = 0. 9996 ）. The mean reeovery of stryehnine was 97.94 % （ RSD = 1.0 %, n = 6 ）. Conclusion： the developed HPLC method was simple, accurate and rapid, and suitable for monitoring of strychnine in Nux Vomiea powder.

关 键 词：高效液相色谱法 士的宁 马钱子散 含量测定 

分 类 号：R927.2[医药卫生—药学]