高压氧对2型糖尿病大鼠降糖作用的机制研究  

Protective effect of hyperbaric oxygen on the reduction of glucose in Goto.Kakizaki rats with type II diabetes mellitus

作　　者：邵贵强 曹秀琴 周玮丽 沈佳伟 史莉华[2] 宋金波[2] 陆海生[2] 

机构地区：[1]闵行区吴泾医院,上海200241 [2]复旦大学附属上海市第五人民医院

出　　处：《中华航海医学与高气压医学杂志》2012年第1期32-36,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：上海市闵行区自然科学研究计划课题（2008MH042）

摘　　要：目的观察高压氧（hyperbaricoxygen，HBO）对2型糖尿病GK大鼠的降糖作用并探讨其作用机制。方法选取5个月龄SPF级雄性GK大鼠72只（空腹血糖均在16．7mmol／L以上），采用数字表法随机分为3组，即模型组、二甲双胍组、HBO组，每组再分成3个亚组，每组均为8只。另取24只Wistar大鼠为正常对照组，每次取8只用于3个不同的实验。HBO组每天行HBO治疗：0．15MPa纯氧，稳压吸氧30min；二甲双胍组按250mg／（kg·d）用二甲双胍混悬液灌胃；正常对照组、模型组、HBO组每天按5ml／（kg·d）灌服纯净水。1～3周后测定空腹血糖、葡萄糖转运蛋白含量。取1只Wistar大鼠、2只HBO组大鼠进行72h血糖动态监测。所有大鼠隔日测定禁食4h后血糖，每组取2只大鼠电镜下观察胰腺组织中胰岛B细胞超微结构。结果GK大鼠二甲双胍组第7天血糖为（9．71±2．07）mmol／L，HBO组第7天血糖为（9．79±2．12）mmol／L，模型组血糖为（9．99±2．31）mmol／L，对照组血糖为（4．90±0．56）mmol／L，各组空腹血糖值比较差异无统计学意义（P〉0．05）；治疗第7天时，对照组血清葡萄糖转运蛋白浓度为（7．12±2．11）ng／L，模型组为（3．98±1．25）ng／L，二甲双胍组为（4．56±1．76）ng／L，HBO组为（4．37±0．68）ng／L，各组比较差异有统计学意义（P〈0．01）；治疗第14天时，对照组血清葡萄糖转运蛋白浓度为（7．06±0．54）ng／L，模型组为（3．99±0．85）ng／L，二甲双胍组为（5．06±0．77）ng／L，对照组与模型组、二甲双胍组比较差异有统计学意义（P〈0．01）。结论HBO可能通过提高葡萄糖转运蛋白水平、改善胰岛B细胞结构与功能而起到降低血糖的作用。Objective To observe the effect of hyperbaric oxygen （HBO） on the reduction of glucose in Goto-Kakizaki （GK） rats with type II diabetes mellitus, and to further explore its mechanism involved. Methods Seventy-two 5-month SPF male GK rats were randomly assigned to 3 groups： the model group, the metformin hydrochloride group and the HBO group, with each group further divided into 3 subgroups. Another 24 male healthy 5-month Wistar rats were chosen as the normal control group. The rats in the HBO group received HBO treatment by inhaling pure oxygen under a constant pressure of 0.15 MPa for 30 minutes, while the rats in group 2 were treated with gastric feeding of metformin hydrochloride 250 mg/（kg · d） once a day. At the same time, all the rats in the control group, the model group and the HBO group had gastric feeding of purified water 5 ml/（ kg · d） once a day. The levels of fasting blood glucose and glucose transport protein （ GLUT-I ） of all the rats were measured respectively after 1,2 and 3 weeks of treatment. Moreover, one Wistar rat in the control group and two in the HBO group were chosen to have dynamic glucose monitoring for 72 hours.Glucose level of all the animals in the 4 groups were measured 4 hours after fasting, every other day, and 2 rats were chosen from each group for observation of the changes in the ultrastructure of islet B ceils in the pancreas under the electron microscope. Results Blood glucose level of the GK rats in the mefformin hydrochloride group on day 7 was （9.71± 2.07 ） mmol/L, that of the rats in the HBO group on day 7 was （9.79± 2.12） mmol/L, that of the rats in the model group on day 7 was（9.99 ±2.31 ）mmol/L, and that of the rats in control group was （4.90±0. 56） mmol/L. No statistical significance could be noted, when comparisons were made between the groups （ P 〉 0.05 ）. Following treatment on d 7, the level of serum glucose transport protein of the control group was （7.12± 2.11 ） ng/L, that of the model group was （3.98 �

关 键 词：高压氧 2型糖尿病 葡萄糖转运蛋白 动态血糖监测 

分 类 号：R587.1[医药卫生—内分泌]