甘氨酸预处理对中华羊茅热胁迫下幼苗生长和氧化损伤的影响(英文)  

Effects of glycine pretreatment on the growth and oxidative damage in heat-stressed Festuca sinensis Keng seedlings

作　　者：王文颖[1] 殷恒霞[1] 徐进[2] 刘小京[2] 米琴[1] 杜军华[1] 马玲玲[1] 周华坤[3] 

机构地区：[1]青海师范大学生命与地理科学学院,西宁810008 [2]中国科学院遗传与发育生物学研究所农业资源中心,石家庄050021 [3]中国科学院西北高原生物研究所,西宁810008

出　　处：《兰州大学学报（自然科学版）》2012年第1期75-78,共4页Journal of Lanzhou University(Natural Sciences)

基　　金：Supported by the National Natural Science Foundation of China(30660120);the Program of Science and Technology Department of Qinghai Province(2010-H-809);the Science and Technology Key Project of the Ministry of Education(209133);the National Basic Research Program of China(973 Program)(2009CB421102);the National Major Special Project on New Varieties Cultivation for Transgenic Organisms(2009ZX08009-130B to J.Xu)

摘　　要：研究了甘氨酸预处理对降低中华羊茅幼苗热胁迫的保护性作用.甘氨酸预处理降低了活性氧水平,保护了热胁迫下幼苗细胞膜的完整性,因而降低了植株根系细胞的死亡率.研究表明:甘氨酸预处理可以提高幼苗超氧化物歧化酶(SOD),过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)的活性.半定量RT-PCR分析表明甘氨酸预处理幼苗中CAT基因的表达比对照高.讨论了抗氧化酶活性在甘氨酸调节热胁迫中的作用.The protective effects of glycine on alleviating the heat stress of Festuca sinensis Keng seedlings were investigated. It was found that glycine pretreatment reduced the reactive oxygen species （ROS） level and protected the plasma membrane integrity of seedlings under heat stress, thus reducing cell death of the roots exposed to high temperature stress. Further study indicated that glycine pretreatment improved the activities of superoxide dismutase （SOD）, catalase （CAT） and ascorbate peroxidase （APX） in the seedlings. Semi-quantitative RT-PCR analysis indicated that the transcripts of CAT gene in glycine pretreated-seedlings were higher compared to untreated control seedlings under heat stress. The involvement of antioxidative enzymes activities in glycine-mitigated heat stress was also discussed.

关 键 词：中华羊茅 甘氨酸 热胁迫 抗氧化酶 

分 类 号：Q945.78[生物学—植物学]