过敏性哮喘患者嗜碱粒细胞CD63和CD203c的表达  

Expressions of CD63 and CD203c in basophils of allergic asthma patients

作　　者：杨玲[1] 李强 王树云[3] 许以平[4] 郭胤仕[4] 姚迪 史桂英[6] 

机构地区：[1]上海交通大学医学院附属新华医院老年科,上海200092 [2]上海市控江医院呼吸科,上海200093 [3]上海交通大学医学院附属新华医院急诊科,上海200092 [4]上海交通大学医学院附属仁济医院呼吸科,上海200001 [5]上海交通大学医学院附属新华医院呼吸科,上海200092 [6]上海交通大学医学院细胞生物学教研室,上海200025

出　　处：《诊断学理论与实践》2012年第1期57-61,共5页Journal of Diagnostics Concepts & Practice

基　　金：上海交通大学医工合作课题(YG2010MS12);上海市卫生局课题(20114310)

摘　　要：目的:探讨过敏性哮喘患者嗜碱粒细胞激活后其CD63和CD203c的表达情况。方法:采用流式细胞术检测过敏性哮喘(哮喘组)、非过敏性哮喘患者(非过敏性哮喘组)及正常对照者(正常对照组)的血嗜碱性粒细胞中CD63和CD203c的表达,并进行比较。结果:以CD123和人类白细胞抗原DR联合设门,可观察到外周血中的嗜碱性粒细胞。与非过敏性哮喘组及正常对照组比较,过敏性哮喘组患者的血嗜碱粒细胞CD63荧光强度显著增加(P<0.01)。经粉尘螨刺激后,与刺激前对照、非过敏性哮喘者及正常对照者比较,过敏性哮喘患者的血嗜碱粒细胞CD203c和CD63荧光强度均显著增加(P<0.01)。嗜碱粒细胞CD203c、CD63表达程度与皮试结果及特异性IgE水平间均存在不同程度的相关性。受试者工作特征曲线分析结果显示,嗜碱粒细胞活化试验的曲线下面积为0.923,高于特异性IgE的曲线下面积(0.876)。结论:采用流式细胞术检测CD63表达来确定活化嗜碱粒细胞是诊断过敏性疾病的一种安全、有效的体外检测方法。Objective To study the expressions of CD63 and CD203c in activated basophils of patients with allergic asthma.Methods Flow cytometry（FCM） was used to measure the expressions of CD63 and CD203c in blood basophils of allergic asthma patients,non-allergic asthma patients and normal controls,and the results were compared.Results Peripheral blood basophils could be selected by using CD123 and HLA-DR as the gate.The median fluorescence intensity（MFI） of CD63 in basophils of allergic asthma patients increased markedly when compared with that of non-allergic asthma patients and normal controls（P〈0.01）.After stimulated with dust mites,the median fluorescence intensity of CD203c and CD63 in basophils of allergic asthma patients increased significantly when compared with that before stimulation and that of non-allergic asthma patients and normal controls（P〈0.01）.The expressions of CD203c and CD63 in basophils were correlated in certain degree with the skin test and specific IgE（sIgE） level.Receiver operating characteristic（ROC） curve analysis showed that the area under curve of basophils activation test（BAT） was 0.923,higher than that of sIgE（0.876）.Conclusions Quantification of activated basophils by detecting CD63 expression with FCM is an effective and safe in vitro method for diagnosing allergic diseases.

关 键 词：过敏性哮喘 嗜碱粒细胞 CD63 CD203c 流式细胞术 

分 类 号：R562.25[医药卫生—呼吸系统]