不同产地、不同采收期和不同部位金钮扣中绿原酸含量比较研究  

Comparative Study on the Content of Chlorogenic Acid in Solanum torvumfrom Different Origin,Different Harvest Time and Different Part

作　　者：何洋[1] 周洪波[1] 刘建雄 李慧珊[1] 李萍[1] 房志坚[1] 

机构地区：[1]广东药学院中山校区,广东中山528458 [2]广东益和堂制药有限公司,广东中山528471

出　　处：《中药材》2012年第1期23-25,共3页Journal of Chinese Medicinal Materials

基　　金：中山市华南现代中医药城(健康医药产业基地)专项发展基金(2007J007)

摘　　要：目的:建立金钮扣药材中有效成分绿原酸HPLC含量测定方法,为其药材质量评价和资源合理利用提供科学依据。方法:采用高效液相色谱法对不同产地、不同采收期和不同部位金钮扣中绿原酸含量进行比较分析。色谱柱为Agela Promosil C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸溶液(10∶90),流速为1.0mL/min,检测波长为327nm,柱温为35℃。结果:绿原酸的浓度在0.1000～200.0μg/mL范围内与峰面积呈良好的线性关系r,=0.9999,平均回收率为99.8%,RSD为0.71%。不同产地、不同采收期和不同部位金钮扣中绿原酸含量存在一定差异。结论:所建立的方法准确可靠、灵敏度高、专属性强,可有效控制金钮扣药材的质量,为金钮扣资源合理利用提供科学依据。Objective ： To develop a HPLC method for determination of chlorogenic acid in Solanum torvum and provide a scientific basis for evaluating the quality and reasonable utilization of the herb. Methods ： HPLC was used to quantitatively determine the chloro- genic acid content in Solanum torvum from different origin, different harvest time and different part. The assay was performed on a Agela Promosil Cls （4. 6 mm - 250 mm,5μm） column and eluted with a mobile phase consisted of acetonitrile -0. 1% phosphoric acid solu- tion at a flow rate of 1.0 mL/min. The column temperature was set at 35 ℃. The detection wavelength was 327 nm. Results ： The cali- bration curve was linear within the range of 0. 1000 - 200.0 tLg/mL （ r = 0. 9999）. The average recoveries were 99. 8% （ RSD = 0. 71% ）. The contents of chlorogenic acid in Solanum torvum were different from different origin,different harvest time and different part. Conclusion ： The method is reliable, accurate and specific. It can be used for quality control of Solanum torvum and reasonable u- tilization of the herb.

关 键 词：金钮扣 高效液相色谱法 绿原酸 

分 类 号：R282.2[医药卫生—中药学]