Glycerol-3-Phosphate Acyltransferase 6 （GPAT6） Is Important for Tapetum Development in Arabidopsis and Plays Multiple Roles in Plant Fertility  

作　　者：Xiao-Chuan Li Jun Zhu Jun Yang Guo-Rui Zhang Wei-Feng Xing Sen Zhang Zhong-Nan Yang 

机构地区：[1]College of Life and Environmental Sciences,Shanghai Normal University,Shanghai 200234,China

出　　处：《Molecular Plant》2012年第1期131-142,共12页分子植物（英文版）

基　　金：This work was supported by grants from the National Science Foundation of China，the National Basic Research Program of China，by the Leading Academic Discipline Project of Shanghai Municipal Education Commission

摘　　要：Glycerol-3-phosphate acyltransferase （GPAT） mediates the initial synthetic step for the formation of glycer- olipids, which act as the major components of biological membranes and the principal stored forms of energy. GPAT6 is a member of the Arabidopsis GPAT family, which is crucial for cutin biosynthesis in sepals and petals. In this work, a func- tional analysis of GPAT6 in anther development and plant fertility was performed. GPAT6 was highly expressed in the tapetum and microspores during anther development. The knockout mutant, gpat6, caused a massive reduction in seed production. This report shows that the ablation of GPAT6 caused defective tapetum development with reduced endoplas- mic reticulum （ER） profiles in the tapetum, which largely led to the abortion of pollen grains and defective pollen wall formation. In addition, pollen germination and pollen tube elongation were affected in the mutant plants. Furthermore, the double mutant analysis showed that GPAT6 and GPAT1 make joint effects on the release of microspores from tetrads and stamen filament elongation. This work shows that GPAT6 plays multiple roles in stamen development and fertility in Arabidopsis.Glycerol-3-phosphate acyltransferase （GPAT） mediates the initial synthetic step for the formation of glycer- olipids, which act as the major components of biological membranes and the principal stored forms of energy. GPAT6 is a member of the Arabidopsis GPAT family, which is crucial for cutin biosynthesis in sepals and petals. In this work, a func- tional analysis of GPAT6 in anther development and plant fertility was performed. GPAT6 was highly expressed in the tapetum and microspores during anther development. The knockout mutant, gpat6, caused a massive reduction in seed production. This report shows that the ablation of GPAT6 caused defective tapetum development with reduced endoplas- mic reticulum （ER） profiles in the tapetum, which largely led to the abortion of pollen grains and defective pollen wall formation. In addition, pollen germination and pollen tube elongation were affected in the mutant plants. Furthermore, the double mutant analysis showed that GPAT6 and GPAT1 make joint effects on the release of microspores from tetrads and stamen filament elongation. This work shows that GPAT6 plays multiple roles in stamen development and fertility in Arabidopsis.

关 键 词：Arabidopsis GPAT6 stamen development TAPETUM endoplasmic reticulum exine and pollen coat glycerolipid. 

分 类 号：S512.1[农业科学—作物学] TQ464.8[化学工程—制药化工]