A Gain-of-Function Mutation in OslAA11 Affects Lateral Root Development in Rice  

作　　者：Zhen-Xing Zhu Yu Liu Shao-Jun Liu Chuan-Zao Mao Yun-Rong Wu Ping Wu 

机构地区：[1]State Key Laboratory of Plant Physiology and Biochemistry,Zhejiang University,Hangzhou 310058,People's Republic of China [2]College of Life Sciences,Zi Jin Gang Campus,Zhejiang University,866 Yu Hang Tang Road,Hangzhou,310058,People's Republic of China

出　　处：《Molecular Plant》2012年第1期154-161,共8页分子植物（英文版）

摘　　要：Lateral roots are important to plants for the uptake of nutrients and water. Several members of the Aux/IAA family have been shown to play crucial roles in lateral root development. Here, a member of the rice Aux/IAA family genes, OslAA 11 （LOC_Os03g43400）, was isolated from a rice mutant defective in lateral root development. The gain-of-function mutation in OslAAll strictly blocks the initiation of lateral root primordia, but it does not affect crown root development. The expression of OslAAll is defined in root tips, lateral root caps, steles, and lateral root primordia. The auxin reporter DR5-GUS （^-glucuronidase） was expressed at lower levels in the mutant than in wild-type, indicating that OslAAll is involved in auxin signaling in root caps. The transcript abundance of both OsPINlb and OsPIN10a was diminished in root tips of the Osiaa 11 mutant. Taken together, the results indicate that the gain-of-function mutation in OslAA 11 caused the inhibition of lateral root development in rice.Lateral roots are important to plants for the uptake of nutrients and water. Several members of the Aux/IAA family have been shown to play crucial roles in lateral root development. Here, a member of the rice Aux/IAA family genes, OslAA 11 （LOC_Os03g43400）, was isolated from a rice mutant defective in lateral root development. The gain-of-function mutation in OslAAll strictly blocks the initiation of lateral root primordia, but it does not affect crown root development. The expression of OslAAll is defined in root tips, lateral root caps, steles, and lateral root primordia. The auxin reporter DR5-GUS （^-glucuronidase） was expressed at lower levels in the mutant than in wild-type, indicating that OslAAll is involved in auxin signaling in root caps. The transcript abundance of both OsPINlb and OsPIN10a was diminished in root tips of the Osiaa 11 mutant. Taken together, the results indicate that the gain-of-function mutation in OslAA 11 caused the inhibition of lateral root development in rice.

关 键 词：Oryza sativa L. OslAAll lateral root auxin signaling. 

分 类 号：S511[农业科学—作物学] S631.201