The Arabidopsis Transcription Factor AtTCP15 Regulates Endoreduplication by Modulating Expression of Key Cell-cycle Genes  

作　　者：Zi-Yu Li Bin Li Ai-Wu Dong 

机构地区：[1]State Key Laboratory of Genetic Engineering,Institute of Plant Biology,Department of Biochemistry,School of Life Sciences,Fudan University,Shanghai200433,China

出　　处：《Molecular Plant》2012年第1期270-280,共11页分子植物（英文版）

摘　　要：Plant cells frequently undergo endoreduplication, a modified cell cycle in which genome is repeatedly rep- licated without cytokinesis. As the key step to achieve final size and function for cells, endoreduplication is prevalent during plant development. However, mechanisms to control the balance between endoreduplication and mitotic cell di- vision are still poorly understood. Here, we show that the Arabidopsis TCP （CINCINNATA-like TEOSINTE BRANCHED1~ CYCLOIDEA-_PCF）-family transcription factor gene AtTCP15 is expressed in trichomes, as well as in rapidly dividing and vascular tissues. Expression of AtTCP15SRDX, AtTCP15 fused with a SRDX repressor domain, induces extra endoredupli- cation in trichomes and cotyledon cells in transgenic Arabidopsis. On the contrary, overexpression of AtTCP15 suppresses endoreduplication in trichomes and other examined cells. Misregulation of AtTCP15 affects the expression of several im- portant genes involved in cell-cycle regulation. AtTCP15 protein binds directly to the promoter regions of CYCA2;3 and RETINOBLASTOMA-RELATED （RBR） genes, which play key roles in endoreduplication. Taken together, AtTCP15 plays an important role in regulating endoreduplication during Arabidopsis development.Plant cells frequently undergo endoreduplication, a modified cell cycle in which genome is repeatedly rep- licated without cytokinesis. As the key step to achieve final size and function for cells, endoreduplication is prevalent during plant development. However, mechanisms to control the balance between endoreduplication and mitotic cell di- vision are still poorly understood. Here, we show that the Arabidopsis TCP （CINCINNATA-like TEOSINTE BRANCHED1~ CYCLOIDEA-_PCF）-family transcription factor gene AtTCP15 is expressed in trichomes, as well as in rapidly dividing and vascular tissues. Expression of AtTCP15SRDX, AtTCP15 fused with a SRDX repressor domain, induces extra endoredupli- cation in trichomes and cotyledon cells in transgenic Arabidopsis. On the contrary, overexpression of AtTCP15 suppresses endoreduplication in trichomes and other examined cells. Misregulation of AtTCP15 affects the expression of several im- portant genes involved in cell-cycle regulation. AtTCP15 protein binds directly to the promoter regions of CYCA2;3 and RETINOBLASTOMA-RELATED （RBR） genes, which play key roles in endoreduplication. Taken together, AtTCP15 plays an important role in regulating endoreduplication during Arabidopsis development.

关 键 词：ARABIDOPSIS TCP transcription factor ENDOREDUPLICATION cell cycle gene expression. 

分 类 号：Q943.2[生物学—植物学] Q253