慢性难治性骨髓炎动物模型制备与鉴定  

Preparation and identification of chronic and refractory osteomyelitis animal model

作　　者：黄金亮[1] 唐辉[1] 范新宇[1] 徐永清[1] 

机构地区：[1]成都军区昆明总医院〈昆明医学院临床教学医院〉全军骨科中心,昆明650032

出　　处：《西南国防医药》2012年第3期236-239,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：全军医学科学技术研究"十一五"计划科技攻关项目(08G035)

摘　　要：目的探讨慢性难治性骨髓炎动物模型制备与鉴定的实验方法。方法根据文献方法制备兔的慢性骨髓炎模型,模型确定后,给予尽可能彻底的清创及敏感抗生素治疗2 w,再观察临床表现、大体观、X线表现以及行骨髓组织细菌培养、组织学观察,最后以组织学观察为基础,确定慢性难治性骨髓炎模型的建立。结果 10只局部出现软组织肿块,20只动物X线片提示局部软组织肿胀影、骨质增生、低密度空洞影;25只骨髓组织细菌培养阳性,21只组织学观察呈慢性炎症改变。结论 21只实验动物慢性难治性骨髓炎模型制备成功,证实此法可成功制备慢性难治性骨髓炎动物模型,成功率为56.8%。Objective To discuss the experimental method of preparation and identification of chronic and refractory osteomyelitis animal model.Methods The rabbit’s chronic osteomyelitis model was prepared based on literature method.After the model was determined,the model animals received debridement and sensitive antibiotic treatment for 2 w.Clinical and X-ray manifestation were observed.Myeloid tissue bacterial culture and histological observation were carried out.At last,based on the histological observation,the establishment of chronic and refractory osteomyelitis model was determined.Results Ten animals had local soft tissue lump,and X-ray film showed local soft tissue swelling shadow,hyperosteogeny and low-density cavity shadow in 20 ones.The results of myeloid tissue bacterial cultures in 25 ones were positive.The histological observation of 21 ones showed chronic inflammatory changes.Conclusion The chronic and refractory osteomyelitis models of twenty one experimental animals are prepared successfully,which verifies that this method can successfully prepare animal models of chronic refractory osteomyelitis with an achievement ratio of 56.8%.

关 键 词：慢性难治性骨髓炎 动物模型 兔 

分 类 号：R681.2[医药卫生—骨科学]