耐复方新诺明香港海鸥型菌中Ⅰ类整合子、磺胺类耐药基因(sul1、sul2)的检测  

Detection of the classⅠintegrons,sul1 and sul2 genes in trimethoprim-sulfamethoxazole resistantLaribacter hongkongensis isolates

作　　者：陈冰[1] 冯嘉丽[1] 李美霞[2] 朱江峰[1] 魏耀红[2] 张欧[1] 康燕[2] 俞守义[1] 陈清[1] 胡静[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院,广州510515 [2]广州市疾病预防控制中心,广州510030

出　　处：《中国人兽共患病学报》2012年第3期223-225,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金项目(No.30700686);广州市医药卫生科技项目(2009-2di-19)

摘　　要：目的了解香港海鸥型菌淡水鱼与蛙来源分离株磺胺类耐药性以及Ⅰ类整合子介导耐药的情况。方法采用K-B纸片扩散法检测香港海鸥菌耐复方新诺明药物菌株,PCR方法扩增耐药菌株sul1、sul2和Ⅰ类整合子基因。结果 128株香港海鸥菌中共有20株耐复方新诺明药物,耐药率15.6%;20株耐药菌株中sul1阳性率90%,sul2阳性率50%,Ⅰ类整合子携带率80%。结论淡水鱼与蛙来源香港海鸥型菌菌耐磺胺的机制可能与Ⅰ类整合子、sul1、sul2基因有关。The objective was to study the occurrence of sulphonamide resistance genes and class Ⅰ integrons in Laribacter hongkongensis（LH） isolates.The isolates were obtained from freshwater fishes and frogs.The trimethoprim-sulfamethoxazole（SXT） agent susceptibility was carried out by the K-B disk diffusion method,and all sulphonamide-resistant isolates were investigated for the presence of sul1,sul2 and class I integron genes by PCR.The 15.6%（20/128） of the Laribacter hongkongensis isolates were resistant to SXT.Among the SXT resistant isolates,sul1,sul2 and class I integron genes were detected out 90%（18/20）,50%（10/20） and 80%（16/20）,respectively.Results indicate that the mechanism of SXT-resistant LH isolates from freshwater fishes and frogs are likely to associate with sul1,sul2 and class I integron genes.

关 键 词：香港海鸥型菌 Ⅰ类整合子 磺胺类耐药基因 

分 类 号：R378[医药卫生—病原生物学]