MMP-9及TIMP-1在支气管扩张症患者气道中的表达  

Expression of MMP-9 and TIMP-1 in the Airway of Bronchiectasis Patients

作　　者：金前[1] 张影清 李国平[1] 许先荣[1] 周颖[1] 

机构地区：[1]浙江省立同德医院呼吸科,杭州310012 [2]嘉兴市第一医院呼吸科,314000

出　　处：《医学研究杂志》2012年第2期64-67,共4页Journal of Medical Research

基　　金：浙江省医药卫生科学研究基金资助项目(2008B033)

摘　　要：目的研究基质金属蛋白酶-9(MMP-9)及其天然抑制因子金属蛋白酶组织抑制因子-1(TIMP-1)在支气管扩张症患者气道中的表达情况,并分析TIMP-1/MMP-9比值失衡情况与支气管扩张症发病及病情进展的关系。方法本实验采用病例对照研究,分设支气管扩张症组、肺炎组及正常对照组,而支气管扩张症组又分为轻、中、重3个亚组。收集支气管肺泡灌洗液,ELISA法检测其中MMP-9及TIMP-1的浓度,并计算TIMP-1/MMP-9比值,同时取气管内膜组织,免疫组化法检测其中MMP-9及TIMP-1的表达。结果 MMP-9在支气管扩张症组的支气管肺泡灌洗液中明显升高(P=0.000),但TIMP-1却无明显升高,TIMP-1/MMP-9比值明显低于肺炎组及正常对照组(均为P=0.000);MMP-9在支气管扩张症患者中随严重程度的增加其浓度亦升高,但TIMP-1却无同步上升,TIMP-1/MMP-9比值重度组较轻度组有明显下降(P=0.005)。结论BE患者中存在TIMP-1/MMP-9比值失衡,且随着病情进展,该比值的失衡情况逐渐加重,提示TIMP-1/MMP-9比值失衡在BE的疾病发生及病情进展中有着重要作用。Objective To study the expression of matrix metalloproteinase-9（MMP-9） and tissue inhibitor of metalloproteinase-1（TIMP-1） in the airway of bronchiectasis patients,and analysis the relationship of TIMP-1/MMP-9 ratio with the generation and progression of bronchiectasis.Methods We adopted the case-control study,and established the bronchiectasis group,the pneumonia group and the normal group.According to the severity of disease,the bronchiectasis group was divided into the mild group,the middle group and the severe group.We acquired the bronchoalveolar lavage fluid（BALF） of these subjects,and tested the concentration of MMP-9 and TIMP-1 with ELISA,then calculated the ratio of TIMP-1/MMP-9.Also,we acquired the tissue of bronchic endomembrane derived from biopsy and operation,and tested the expression of MMP-9 and TIMP-1 with immunohistochemical method.Results The concentration of MMP-9 in BALF of BE was much higher than that of other groups（P=0.000）,but the concentration of TIMP-1 was not so high in BALF of BE.TIMP-1/MMP-9 ratio of BE group was much lower than that of other groups（both P=0.000）.In the BE group,the concentration of MMP-9 increased as the severity increasing,but the concentration of TIMP-1 didn′t increase as the severity increasing.TIMP-1/MMP-9 ratio was much lower in the severe group than that in the mild group（P=0.005）.Conclusion There is obviously unbalance of MMP-9 and TIMP-1 expression in BE patients.With the disease progressing,this unbalance also deteriorates,which indicates that the unbalance of MMP-9 and TIMP-1 expression has a important role in the generation and progression of BE.

关 键 词：支气管扩张症 基质金属蛋白酶-9 金属蛋白酶组织抑制因子-1 

分 类 号：R562.22[医药卫生—呼吸系统]