野菊花中绿原酸与木犀草苷同时超声提取条件优化  

Optimization of Ultrasonic-assisted Extraction of Chlorogenic Acid and Luteoloside from Chrysanthemum indicum L.

作　　者：何小珍[1] 蒋军辉[2] 徐小娜[3] 喻翠云[1] 贺冬秀[1] 

机构地区：[1]南华大学药学系,湖南衡阳421001 [2]南华大学化学化工学院,湖南衡阳421001 [3]南华大学公共卫生学院,湖南衡阳421001

出　　处：《广州化工》2012年第5期8-9,16,共3页GuangZhou Chemical Industry

基　　金：国家自然科学基金资助项目(81102409);湖南省教育厅基金资助项目(10C1179)

摘　　要：利用超声波辅助系统从野菊花中同时提取绿原酸和木犀草苷,通过正交试验设计考察料液比、提取时间、乙醇浓度、提取温度对绿原酸和木犀草苷含量的影响。试验结果显示野菊花中绿原酸和木犀草苷同时提取的最佳工艺条件为:75%乙醇水溶液、料液比1∶400、提取时间90 min、提取温度50℃。在最佳提取条件下野菊花中绿原酸和木犀草苷得率分别可达(8.94±0.03)mg/g和(2.97±0.04)mg/g。The simultaneous extraction of chlorogenic acid and luteoloside from Chrysanthemum indicum L.by ultrasonic-assisted extraction technology was studied.The effects of several influential parameters of the ultrasonic-assisted extraction procedure were investigated by orthogonal array design experiment.The results showed optimum extract conditions were that the extraction solvent was 75% ethanol,the ratio of material to solvent was 1：400,the extraction time was 90 min and the extraction temperature was 50 ℃.Under the optimum conditions of ultrasonic-assisted extraction,the yields of chlorogenic acid and luteoloside were（8.94±0.03） mg/g and（2.97±0.04） mg/g,respectively.

关 键 词：野菊花 绿原酸 木犀草苷 超声提取 正交设计 

分 类 号：R282.6[医药卫生—中药学]