2010年度北京地区儿童手足口病住院患者病原学分布分析  

Etiology of hand, foot and mouth disease in Beijing in 2010

作　　者：李洪杰[1] 庞琳[2] 王琦[3] 曾慧慧[2] 何明[2] 张锦前[3] 刘顺爱[3] 李兴旺[2] 成军[1] 

机构地区：[1]北京大学北京地坛医院教学医院,北京100015 [2]首都医科大学附属北京地坛医院感染性疾病诊疗中心 [3]首都医科大学附属北京地坛医院感染性疾病诊疗中心传染病研究所

出　　处：《中华实验和临床感染病杂志（电子版）》2012年第1期9-12,共4页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：国家科技部"十一五"专项课题(2009ZX10004-105);北京市科委科技项目(D09050703560908)

摘　　要：目的了解2010年北京地区儿童手足口病住院患者的病原体分布情况,为手足口病的诊断、治疗及预防提供依据。方法本研究共采集337例手足口病住院患儿的咽拭子标本,采用肠道病毒(EV)通用型、柯萨奇病毒A16(CA16)型和肠道病毒71(EV71)型核酸检测试剂盒,应用实时逆转录-聚合酶链反应(RT-PCR)法进行检测。结果肠道病毒阳性标本占63.20%(213/337)。其中,EV71阳性标本占40.36%(136/337);CA16阳性标本占5.64%(19/337);其他未分型肠道病毒阳性标本占17.21%(58/337)。结论 2010年北京地区儿童手足口病住院患者的病原学分布以EV71为主,非CA16和非EV71型肠道病毒感染率已显著高于CA16型肠道病毒感染。Objective To investigate the pathogen distribution of patients with hand, foot and mouth diseases （HFMD） hospitalized in division of pediatrics, Beijing Ditan Hospital, Capital Medical University, in 2010. Methods Real-time fluorescence quantitative RT-PCR kits with three reagents： universal enterovirus primer, Coxsackievirus A16 （CA16） primer and enterovirus 71 （EV71） primer were used to detect the samples. Results The enterovirus-posifive rate was 63.20% （213/337）. Among this, the positive rates of enterovirus 71, Coxsackievirus A-16 and other types of enterovirus were 40. 36% （136/337）, 5.46%（19/337） and 17.21%（58/337）, respectively. Conclusions In 2010, the etiology of children with hand, foot and mouth disease hospitalized in our hospital was dominated by enterovirus 71 （EV71）. The infection rates of enterovirus except enterovirus 71 and Coxsackievirus A16 are main pathogens. Non-CA16, non-EV71 virus infection were significant higher than Coxsackievirus A16 infection.

关 键 词：手足口病 逆转录聚合酶链反应 肠道病毒属 柯萨奇病毒属 

分 类 号：R725.1[医药卫生—儿科]