核磁共振波谱法测定体外培养成骨细胞中氟含量的研究  

Detection of fluoride ion concentration in osteoblasts in vitro by nuclear magnetic resonance spectroscopy

作　　者：钟近洁[1] 钟近艺[2] 刘景全[2] 白生宾[1] 范淑玲[1] 张亚楼[1] 冯树梅[1] 秦纹[1] 陈龙[1] 李甜[1] 廖礼彬[1] 刘开泰[3] 

机构地区：[1]新疆医科大学组织胚胎学教研室,乌鲁木齐830011 [2]中国人民解放军防化研究院第六所,乌鲁木齐830011 [3]中国疾病预防控制中心教育培训处,乌鲁木齐830011

出　　处：《中国地方病学杂志》2012年第2期147-150,共4页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金（81160331、81102084、81000844）

摘　　要：目的了解染氟后体外培养成骨细胞中氟的实际含量，以及氟在成骨细胞的分布情况，为探讨氟对成骨细胞损伤的研究提供基础资料。方法体外培养成骨细胞分别染氟0、5、lO、20、40mg／L，在培养第3、10、30天时制备待测样品，每组6份。采用核磁共振波谱法，对染氟后体外培养小鼠成骨细胞的细胞质和细胞核中氟含量进行定量检测。结果①培养第3天，染氟0、5、10、20、40mg／L组细胞质中氟含量分别为（0．83±0．65）、（0．54±0．23）、（0．65±0．77）、（0．59±0．87）、（3．64±1．21）mg／L，其中染氟40mg／L组明显高于0、5mg／L组（P均〈0．05）。②培养第10天，染氟10、20、40mg／L组细胞质中氟含量[（4．03±1．23）、（3．66±0．98）、（6．26±2．10）mg／L]高于0、5mg／L组[（0．78±0．75）、（2．69±0．89）mg／L，P均〈0．05]，染氟20、40mg／L组细胞核中氟含量[（1．63±1．19）、（2．17±1．21）mg／L]高于0、5mg／L组[（0．65±0．46）、（1．57±0．33）m∥L，P均〈0．05]。③培养第30天，染氟10、20、40mg／L组细胞质中氟含量[（3．99±0．84）、（4．33±1．67）、（5．80±1．38）mg／L]高于0、5mg／L组[（0．88±0．44）、（2．84±0．43），P均〈0．05]，而染氟20、40mg／L组细胞核中氟含量[（3．33±1．46）、（3．53±1．22）mg／L]明显高于0、5mg／L组[（0．70±0．66）、（1．99±0．76）mg／L，P均〈0．05]。结论当成骨细胞处于氟环境时．氟离子很快进入细胞内，并很快在细胞核内积聚．表现出氟与骨组织的特殊亲和性。成骨细胞内氟离子量随着接触时间和接触剂量的增加而升高。Objective To detect the concentration and distribution of fluoride ions in osteoblasts exposed to fluoride in vitro culture, and to provide basic information for studying the effect of fluoride on osteoblast injury. Methods In vitro cultured osteoblasts were exposed to 0, 5, 10, 20, 40 mg/L fluoride for 3, 10, 30 d （n = 6）, respectively. Concentration and distribution of fluoride ions in the cytoplasm and the nucleus of these osteoblasts were determined by nuclear magnetic resonance spectroscopy. Results ①After cultured for 3 d, fluoride ion content of the bone cytoplasm exposed to different concentrations of fluoride 0, 5, 10, 20, 40 mg/L were （0.83± 0.65）, （0.54±0.23）, （0.65 ±0.77）, （0.59 ±0.87）, （3.64 ±1.21）mg/L, respectively, and the values of exposed to 40 mg/L fluoride group was significantly higher than that of exposed to 0, 5 mg/L groups （all P 〈 0.05）. ②after cultured for 10 d, the composition of the fluoride ion in cytoplasm of exposed to fluoride 10, 20, 40 mg/L groups were （4.03 ± 1.23）,（3.66 ±0.98）, （6.26 ±2.10）mg/L, respectively, which were higher than that of exposed to 0, 5 mg/L groups [ （0.78 ：t： 0.75）, （2.69± 0.89）mg/L, respectively, all P 〈 0.05]. Of fluoride 20, 40 mg/L groups,the composition of the fluoride ion in nucleus were （1.63± 1.19）, （2.17 ± 1.21）mg/L, respectively, which were higher than that of 0, 5 mg/L groups [ （0.65±0.46）, （ 1.57 ± 0.33） mg/L, all P 〈 0.05]. ③After cultured for 30 d, of the exposed to fluoride 10, 20, 40 mg/L groups, the composition of the fluoride ion in cytoplasm were （3.99±0.84）, （4.33 ± 1.67）, （5.80 ± 1.38）mg/L, respectively, which were higher than that of 0, 5 mg/L groups[ （0.88 ± 0.44）, （2.84 ± 0.43）mg/L, all P 〈 0.05]. The composition of the fluoride ion in nucleus of the fluoride 20, 40 mg/L groups were （3.33 ±1.46）, （3.53 ± 1.22）mg/L, respectively, which were significantly higher than that of 0, 5 mg/L groups [（0.70 ± 0.66�

关 键 词：核磁共振波谱法 成骨细胞 氟 

分 类 号：R599[医药卫生—内科学]