Plackett-Burman实验设计联用星点设计效应面法优选大黄地上部位蒽醌类成分提取工艺  

Extraction Optimization of Anthraquinone from Aerial Part of Rheum officinale Baill Based on Plackett-Burman Design Combined with CCD response Surfa-ce Methodology

作　　者：谭玉柱[1] 童婷婷[1] 谢运飞[1] 赵高琼[1] 董小萍[1] 

机构地区：[1]成都中医药大学,成都611137

出　　处：《中国药学杂志》2012年第6期410-414,共5页Chinese Pharmaceutical Journal

基　　金：国家十一五科技支撑计划地方专项(2009BAI84B00)

摘　　要：目的运用Plackett-Burman实验设计联用星点设计(central composite design,CCD)效应面法,考察各影响因素显著性,筛选出大黄地上部位(aerial part of Rheum officinale Baill,APOR)总蒽醌及大黄素提取的最佳工艺,为非药用部位的开发与应用提供依据。方法运用Plackett-Burman实验设计筛选主要影响因素,采用CCD-效应面积法优选APOR蒽醌提取工艺。以乙醇体积分数、提取时间、料液比为自变量,总蒽醌和大黄素含量为因变量,通过对自变量与因变量的完全二次响应曲面的回归拟合,利用三维曲面图直观分析APOR蒽醌提取最佳工艺,并进行预测分析。结果总蒽醌的最佳提取工艺为:乙醇体积分数67.25%,时间90 min,料液比28∶1;大黄素的最佳提取工艺为:乙醇体积分数85%,时间90 min,料液比40∶1。结论利用Plackett-Burman实验设计联用CCD确定了APOR总蒽醌和大黄素的提取工艺,该方法简便,精度更高、重现性好、预测性强。OBJECTIVE To optimize the process of extacting effective constituents from aerial part of Rheum officinale Baill by Plackett-Burman design combined with CCD response surface methodology. METHODS In Plackett-Burman combined with CCD re- sponse surface design, the independent variables were concentration of ethanol, extraction time, and solvent fold. The dependent varia- bles were contents of total anthraquinone and rheum emodin. RESULTS The optimal extraction process of total anthraquinone was as follows： 67.25% ethanol, 90 min for reflux per time, 28：1 fold of solvent and 2 times of extraction; the optimal extraction process of emodin was 85% ethanol, 90 min for reflux per time,40 fold of solvent and 2 times of extration. CONCLUSION The optimal process is simpleand convenient for extracting aerial part of Rheum officinale Baill with high precision and predictability.

关 键 词：药用大黄 总蒽醌 大黄素 Plackett—Burman 星点设计 提取工艺 

分 类 号：R283[医药卫生—中药学]