孕激素受体在雌性山羊肠系膜后神经节的表达  

Expression of Progestogen Receptor in Inferior Mesenteric Ganglion of Female Goat

作　　者：吴卫妮[1] 任妮[1] 王红梅[1] 徐永平[1] 范洁[1] 王志豪[1] 明佳[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《中国农业科学》2012年第5期1017-1022,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(31072184);陕西省农业推广专项(14220201)

摘　　要：【目的】探究肠系膜后神经节是否对孕激素具有反应性。【方法】试验选取雌山羊肠系膜后神经节,经免疫组织化学SP法,检测孕激素受体(progestogen receptor,PR)在肠系膜后神经节的表达及分布情况。【结果】孕激素受体阳性神经元在雌山羊肠系膜后神经节广泛存在,强阳性产物存在于神经元胞核中,阳性产物存在于整个神经元胞体以及神经元核仁中。在神经节中神经纤维,微血管及毛细血管内皮细胞中也出现了阳性产物。【结论】在雌山羊肠系膜后神经节中存在孕激素受体。【Objective】 The purpose of the present research was to explore whether inferior mesenteric ganglion(IMG) of female goat has reactivity to the progestogen.【Method】 Based on immunohistochemical staining and RT-PCR technique,the expression and distribution of progestogen receptor(PR) in the IMG of female goat were detected.【Result】 The PR immunoreactive products locate widely in the IMG of female goat,they have high staining intensity in the nucleolus,ecumenic staining intensity in the whole cytoplasm and nucleoli.The immunoreactive products also exist in other composition of the IGM.【Conclusion】It was demonstrated that PR existed in the female goat IGM.

关 键 词：肠系膜后神经节 孕激素受体 免疫组织化学SP法 雌山羊 

分 类 号：S827[农业科学—畜牧学]