支架荷载的骨髓间充质干细胞的分化实验研究  

The differentiation of rat bone marrow mesenchymal stem cells on scafold

作　　者：贺斌 王明刚 赵李平 钟晓红 徐飞 

机构地区：[1]安徽医科大学附属省立医院烧伤整形外科,合肥230001

出　　处：《安徽医科大学学报》2012年第4期400-404,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:11040606M161);安徽高校省级科研基金(编号:KJ2011Z205;KJ2008B297);安徽省卫生厅课题(编号:09A028)

摘　　要：目的探讨大鼠骨髓间充质干细胞(BMSCs)在改性聚乳酸-Ⅰ型胶原复合支架上向表皮干细胞分化的可行性。方法体外诱导改性聚乳酸-Ⅰ型胶原复合支架荷载的大鼠骨髓间充质干细胞向表皮干细胞分化,免疫组织化学法检测表皮干细胞标志物CK19和整合素β1的表达。结果诱导液作用2周后,免疫组织化学法检测到改性聚乳酸-Ⅰ型胶原复合支架荷载的大鼠BMSCs中大量阳性双表达CK19和整合素β1。结论改性聚乳酸-Ⅰ型胶原复合支架荷载的大鼠BMSCs仍具有向表皮干细胞分化的潜能,此支架具备作为组织工程皮肤支架材料的条件。Objective To investigate the feasibility of Rat bone marrow mesenchymal stem cells（BMSCs） differeniated into epidermal cells on modified poly L acid and type collagen Ⅰ composites scafold（PLA-Ⅰ）.Methods Differentiation of rat BMSCs on PLA-Ⅰ into epidermal stem cells in vitro by EGF induced cell culture solution.Two-signed of immunohistochemistry identifed CK19 and intergrin β1 markers epidermal stem cells.Results After two weeks of EGF induced cell culture solution,expression of CK19 and intergrin β1 was highly at rat BMSCs on PLA-Ⅰ.Conclusion Rat BMSCs still have the potential to differentiate into epidermal cells on PLA-Ⅰ,which can be used as a vehicle for tissue engineering skin.

关 键 词：改性聚乳酸 Ⅰ型胶原 支架 干细胞 

分 类 号：R622.1[医药卫生—整形外科] R318.1[医药卫生—外科学]