IL-1β和IL-1RN基因多态性与胃食管反流病相关性的研究  

Relationship between gene polymorphisms of IL-1β or IL-1RNand gastroesophageal reflux disease

作　　者：程鹏[1] 王亚雷[1] 许建明[1] 唐小波[1] 王凯[1] 冯慧[1] 

机构地区：[1]安徽医科大学第一附属医院消化内科,安徽省消化系统疾病重点实验室,合肥230022

出　　处：《安徽医科大学学报》2012年第4期442-446,共5页Acta Universitatis Medicinalis Anhui

摘　　要：目的研究胃食管反流病(GERD)患者白细胞介素1B(IL-1β)基因和白细胞介素1受体拮抗剂(IL-1RN)基因的基因多态性情况,并分析各种基因型与GERD的发病风险、内镜下表现的相关性。方法采用病例对照研究方法,收集154例GERD患者和160例健康人外周静脉血,提取DNA,采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法检测IL-1β-511基因型,采用聚合酶链式反应(PCR)方法测定IL-1RN基因型,比较各种基因型及各等位基因的分布情况。结果 IL-1β-511基因多态性及各等位基因与GERD患病风险均无相关性;IL-1RN*1,2基因型及2等位基因可能增加GERD患病风险(P<0.05);IL-1β-511*T/IL-1RN*1(T1)可能降低GERD患病风险(P<0.05),其他各组基因型差异均无显著性;IL-1β-511-CC基因型及C等位基因频率在NERD组明显高于反流性食管炎组,差异有显著性(P<0.05),在没有Hp感染时,这种差异更明显。结论IL-1β和IL-1RN基因多态性与GERD的患病风险、内镜下表现之间具有相关性。Objective To study the gene polymorphisms of interleukin-1β（IL-1β） and IL-1RN in patientswith gastroesophageal reflux disease（GERD）,and to analysis the relationship between the gene polymorphisms and the risk,the severity at endoscopy of GERD.Methods The gene polymorphisms of IL-1β and IL-1RN were analyzed by PCR-restriction fragment length polymorphism（PCR-RFLP） after extracting the genomic DNA from 154 GERD patients and 160 cases healthy controls.Results ① No significant difference was observed between GERD patients and controls in the gene polymorphisms of IL-1β-511;② The IL-1RN 1,2 genotype and 2 allele were associated with higher risk of GERD than the 1,1 genotype and the 1 allele（P0.05）;③ The IL-1β-511＊T / IL-1RN＊1（＂T1＂） haplotype showed lower risk of GERD（P0.05）;④ In the patients with RE,the IL-1β-511 CC genotype and C allele were associated with lower risk than the TT genotype and the T allele（P0.05）,particularly in the Hp negative patients.Conclusion Gene polymorphisms of IL-1β or IL-1RN are associated with the risk,the severity at endoscopy of GERD.

关 键 词：胃食管反流病 基因多态性 白细胞介素-1B 白细胞介素-1受体拮抗剂基因 

分 类 号：R392.2[医药卫生—免疫学] R573.9[医药卫生—基础医学]