甲基磺酸甲酯处理毛泡桐丛枝病幼苗的形态变化及SSR分析  

Morphological changes of the witches' broom seedlings of Paulownia tomentosa treated with methyl methanesulphonate and SSR analysis

作　　者：曹喜兵[1] 范国强[1] 翟晓巧[1] 

机构地区：[1]河南农业大学泡桐研究所,郑州450002

出　　处：《植物病理学报》2012年第2期214-218,共5页Acta Phytopathologica Sinica

基　　金：国家自然科学基金项目(30271082;30571496);高等学校博士学科点专项科研基金项目(20050466003);中国博士后基金项目(20070410874)

摘　　要：在真核生物中，DNA甲基化可导致基因特异表达、细胞分化和染色体失活等，可在不改变DNA碱基排列顺序的同时，阻断遗传信息的传递，Witches＇ broom is one of the most serious diseases in Paulownia production. Morphological changes of the witches＇ broom seedlings of Paulownia tomentosa treated with methyl methanesulphonate and SSR analysis were investigated in order to further study the relation between the disease and DNA changes. The resuits indicated that the diseased seedlings treated with optimal concentration of MMS might turn into the ones without symptom morphologically, but there still existed the phytoplasma in the seedlings treated with 20 mg·L^-1 MMS. Not only might MMS of which the concentrations were more than 80 mg·L^-1 eliminates the phytoplasma from the seedlings, but also inhibits the seedling growth. DNA base sequences of the healthy, diseased and the diseased-treated with 20 and 100 mg·L^-1 MMS seedlings were the same at SSR level.

关 键 词：SSR分析 泡桐丛枝病 形态变化 甲基磺酸 DNA甲基化 幼苗 甲酯 真核生物 

分 类 号：S661.2[农业科学—果树学]