101份贵州玉米地方种质的SSR遗传多样性分析  

Genetic Diversity of 101 Maize Germplasms from Guizhou Province as Revealed by SSR Markers

作　　者：韩萌[1] 柏光晓[1] 

机构地区：[1]贵州大学农学院,贵州贵阳550025

出　　处：《山地农业生物学报》2012年第1期5-8,共4页Journal of Mountain Agriculture and Biology

基　　金：贵州省公关项目[黔科合NY字(20113014号)]

摘　　要：为了分析101份贵州玉米地方种质的遗传多样性及其之间的遗传关系,利用微卫星分子标记(SSR)技术,从220对SSR引物中筛选出23对差异比较明显的SSR引物进行PCR扩增,共检测出252个等位基因变异,变幅为7～21个,平均为11个。每个位点的多态性信息量(PIC值)分布范围为0.753～0.945,平均为0.857。遗传相异系数变幅在0.722～0.905之间,平均值为0.812。以遗传相异系数0.80为界进行聚类分析,可将材料划分为5个大群,其中来自册亨县的大黄早单独划分在第一类群,与其他4个类群的遗传距离较远。利用SSR进行玉米亲缘关系分析,为贵州地方种质资源系统研究和今后玉米育种提供了理论依据。The genetic diversity of 101 maize germplasms originated from Guizhou Province was analyzed by the SSR molecular markers generated from 23 pairs of stable SSR primers,which screened from 220 pairs.A total of 252 alleles were detected,and the average alleles per SSR loci were 11 with a range from 7 to 21.The Polymorphic Information Content（PIC） for the SSR loci varied from 0.753 to 0.945 with an average of 0.857.Genetic dissimilarity coefficients ranged from 0.722 to 0.905 with an average of 0.812.Taking 0.80 of genetic dissimilarity coefficient as thresheld,the 101 maize germplasms might be divided into five groups by UPGMA method based on SSR markers.The far genetic distance was investigated between the 5th group and other four ones.This separated group was Dahuangzao from Ceheng County.Therefore,the genetic relationships among these maize germplasms by SSR marker may provide a theoretical basis for further breeding.

关 键 词：玉米 地方种质 SSR标记 遗传多样性 聚类分析 

分 类 号：S635.1[农业科学—蔬菜学]