低蛋白合并α-酮酸饮食对被动型Heymann肾炎大鼠肾小球足细胞Nephrin表达的影响  

Effects of low protein diet with α-keto acid on nephrin expression of podocytes in passive heymann nephritis rat

作　　者：王锋[1] 范亚平[1] 达展云[1] 吴建华[1] 张义德[1] 

机构地区：[1]南通大学附属医院,江苏南通226001

出　　处：《山东医药》2012年第1期31-33,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30670986)

摘　　要：目的观察低蛋白合并α-酮酸饮食对被动型Heymann肾炎大鼠肾小球足细胞Nephrin表达的影响。方法将40只SD大鼠分为对照组、模型组(PHN组)、低蛋白饮食组(LPD组)和低蛋白合并α-酮酸饮食组(LK组),对照组经尾静脉注射1 mL/100 g生理盐水,其他三组经尾静脉注射1 mL/100 g新西兰大耳兔抗FX1A血清。对照组、PHN组饮食蛋白占饲料质量的10%,LPD组饮食蛋白占饲料质量的5%,LK组饮食蛋白占饲料质量的5%(其中α-酮酸提供1%的蛋白)。4周后检测各组24 h尿蛋白定量、血清肌酐、TG、TC及血清白蛋白,激光共聚焦显微镜免疫荧光检查大鼠肾小球足细胞Nephrin表达情况。结果激光共聚焦显微镜显示,PHN组、LPD组肾小球内Nephrin荧光强度较对照组明显减弱,且部分节段有缺失;LK组肾小球内Nephrin荧光强度较对照组减弱。结论低蛋白合并α-酮酸饮食对被动型Heymann肾炎大鼠具有肾脏保护作用。To investigate the influence of low protein diet with αketo acid on expression changes of neph- rin of podoeytes in passive heymann nephritis rat. Methods A total of 40 SD rats were divided into control group, PHN group, LPD group and LK group. The rats in control group were injected into vena eaudalis with 1 mL/100 g normal saline, and in other three groups injected into vena caudalis with l mL/100 g antiFX1A serum. 10% normal protein diet was taken in control group and PHB group, 5% low protein diet taken in LPD group, and 5% low protein diet or 4% low protein plus 1% αketo acid diet taken in LK group. After four weeks, twentyfour hours urine protein, serum albumin, serum creatine , serum triglyeeride and serum total eholestorel were observed respectively. Renal exprssions of nephrin from rats were determined by indirect immunofluorescence histochemitry. Results Laser fluorescence intensity displayed that compared to control group, fluorescence intensity of nephrin in glomerular were more significantly decreased in PHN, LPD group, and even part segment depletion; nephrin fluorescence intensity in LK group became damp. Conclusion Low protein diet with αketo acids supplement therapy exhibit renal protective in passive heymann nephritis rats.

关 键 词：肾小球肾炎 膜性 NEPHRIN 膳食 限制蛋白质 Α酮酸 小鼠 

分 类 号：R692.6[医药卫生—泌尿科学]