清心开窍方挥发油对AD大鼠皮质GFAP、Aβ及βAPP表达的影响  

Effect of Qingxin Kaiqiao Prescription and Volatile Oil on the Expression of GFAP、Aβ and βAPP in the Cortex of AD Rats

作　　者：朱未名 胡海燕[2] 陈翔[2] 王文花[2] 张晓艳[2] 楚生辉[2] 

机构地区：[1]温州鹿城精神病院,浙江温州325003 [2]温州医学院,浙江温州325003

出　　处：《中医药学报》2012年第1期13-16,共4页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金资助项目(No.30973780);浙江省自然科学基金资助项目(No.Y2080273);温州市科技局资助项目(No.Y20080106)

摘　　要：目的:探讨清心开窍方挥发油对Aβ25-35杏仁核注射诱导AD大鼠皮质区GFAP、Aβ、βAPP及IL-6表达的影响。方法:雄性SD大鼠40只,随机分为正常组、模型组、安理申组、清心开窍方组和挥发油组。采用杏仁核注射Aβ25-35诱导AD大鼠模型,观察大鼠水迷宫空间记忆能力,并通过免疫组化方法研究皮质区GFAP、Aβ、βAPP及IL-6的表达。结果:清心开窍方和挥发油能改善AD大鼠学习记忆能力,大鼠皮质区GFAP、Aβ、βAPP及IL-6表达水平降低,与对照组比较有显著性差异(P<0.05)。结论:清心开窍方能明显改善AD大鼠学习记忆能力,其机制可能是通过降低皮质GFAP、Aβ、βAPP及IL-6的表达发生作用。Objective：To study the effect of Qingxin Kaiqiao prescription and volatile oil on the expression of GFAP,Aβ and βAPP in the cortex of AD rats.Methods：Forty male SD rats were randomly divided into five groups： the control group,the model group,the Aricept group,the Qingxin Kaiqiao prescription group and the volatile oil group.AD was induced by Aβ25-35 injected into the bilateral amygdala to observe the spatial learning and memony ability and the expression of GFAP,Aβ,βAPP and IL-6by immunohistochemistry in the cortex of the animal.Results： Learning and memory ability of Qingxin Kaiqiao prescription group and volatile oil group were improved,and the expression of GFAP,Aβ,βAPP and IL-6 in the cortex of AD rats decreased.Compared with comparison group,the difference was notable（P0.05）.Conclusion：Qingxin Kaiqiao prescription may obviously improve the learning and memory ability of rats with AD by decreasing the expression of GFAP,Aβ,βAPP and IL-6 in the cortex.

关 键 词：阿尔茨海默病 清心开窍方 胶质纤维酸性蛋白 淀粉样蛋白 

分 类 号：R285.5[医药卫生—中药学]