数字全息显微定量相位成像的实验研究  

Experimental Study on Quantitative Phase Imaging by Digital Holographic Microscopy

作　　者：马利红[1] 王辉[1] 金洪震[1] 李勇[1] 

机构地区：[1]浙江师范大学信息光学研究所,浙江金华321004

出　　处：《中国激光》2012年第3期209-215,共7页Chinese Journal of Lasers

基　　金：国家自然科学基金(60877002);浙江省自然科学基金(Z1080030);浙江省教育厅项目(201016465);金华市科学技术研究项目(2010-1-050);浙江师范大学创新团队项目资助课题

摘　　要：建立了一套预放大式数字全息显微成像系统,通过对样品进行显微放大,实现了高分辨率的定量相位成像;并通过计算机控制相机自动曝光记录序列的数字全息图实现了动态相位成像。用标准样品验证了系统测量的准确性;以活体洋葱表皮细胞和血红细胞为样品,获得了清晰的定量相位像;以置于水环境的草履虫为样品,实现了动态成像。实验结果表明建立的系统可以实现高分辨率的动态定量相位成像,可以应用于生物活体样品的显微研究。Digital holographic microscopy apparatus with pre-magnification has been set up,which can well acquire the quantitative phase images for living biological specimens.In addition,a series of digital holograms can be automatically recorded and movies of holographic phase images of living biological specimens can be created.The accuracy of quantitative imaging is verified by a self-produced stepped transparent sample with known height and refraction.Onion epidermal cells and red blood cells are measured and quantitative high-quality phase images are obtained.The movies of phase images for paramecium are achieved.Experimental results demonstrate that the established system can achieve real-time quantitative high-resolution holographic phase image and can be effectively applied to living biological specimens phase imaging.

关 键 词：全息 数字全息 数字全息显微 定量相位成像 活体生物样品 

分 类 号：TB877.1[一般工业技术—摄影技术]