转Cry1Ab和Cry1Ac融合基因型抗虫水稻对田间二化螟和大螟种群发生动态的影响  

Effects of insect-resistant transgenic Bt rice with a fused Cry1Ab+Cry1Ac gene on population dynamics of the stem borers,Chilo suppressalis and Sesamia inferens,occurring in paddyfield

作　　者：李志毅[1] 隋贺[1] 徐艳博[1] 韩兰芝[2] 陈法军[1] 

机构地区：[1]南京农业大学植物保护学院昆虫系,昆虫信息生态研究室,南京210095 [2]中国农业科学院植物保护研究所,水稻害虫研究组,北京100094

出　　处：《生态学报》2012年第6期1783-1789,共7页Acta Ecologica Sinica

基　　金：转基因生物新品种培育重大专项课题(2009ZX08011-007B;2009ZX08012-005B和2011ZX08012-005);教育部霍英东基金(122033);博士点基金(20070307002)

摘　　要：以Bt水稻华恢1号(Cry1Ac和CryAb融合基因;简称HH1)及其对照亲本明恢63(简称MH63)稻田靶标害虫二化螟Chilosuppressalis和次靶标害虫大螟Sesamia inferens为研究对象,研究了转基因抗虫水稻大田螟虫发生规律及其靶标和次靶标害虫致害力差异。结果表明,Bt水稻及其对照亲本上二化螟或大螟的卵块数量差异不显著,同时,对照亲本上二化螟与大螟的落卵量差异不显著,但Bt水稻上二化螟的落卵量显著大于大螟。与对照亲本相比,Bt水稻上二化螟幼虫发生量显著降低,降幅高达84.9%—100%,但大螟发生量差异不显著;此外,对照亲本上二化螟幼虫发生量显著高于大螟,但Bt水稻上两者差异不显著。同时,Bt水稻上二化螟导致的枯心/白穗率和受害丛率都显著低于其在对照亲本上的致害程度,降幅分别为30.8%—98.3%和11.4%—96.6%,而大螟差异不显著。可见,Bt水稻对靶标害虫二化螟具有较高抗性,而对次靶标害虫大螟的抗性不明显。另一方面,Bt水稻和对照亲本上二化螟导致的枯心/白穗率和受害从率都显著高于大螟。可见,二化螟仍是当前非转基因水稻上的主要害虫,而Bt水稻对二化螟幼虫发生的显著抑制作用以及对大螟幼虫发生的不显著影响,使得其大面积商业化种植下靶标害虫二化螟和次靶标害虫大螟间的竞争替代成为可能。The approval of transgenic Bacillus thuringiensis rice （ ab. , restorer line of cv. Bt Huahui 1 and a hybrid rice line of cv. Bt Sh Bt rice） with doublestacked traits （including a rice anyou gene） by China＇s Ministry of Agricuhure （MOA） on 27 November 2009 was To date, some experiments have been carried out in labs or under controlled Bt rice can perform remarkably well against two targeted lepidopteran pests （ both expressing a fused CrylAb＋CrylAc a momentous day for biotech crops worldwide. environmental conditions, which indicate that e. g. , the stem borer, Chilo suppressalis,leaffolder, Cnaphalocrocis medinalis ）, and simultaneously offers： 1 ） the potential to generate economic benefits due to yield increases, and 2） overall decreases in insecticide use in China. In this study, the field experiments were conducted in order to examine the population dynamics and damage characteristics of two stem borers （ i. e. , target pest, C. suppressalis and a sub-target pest, Sesamia inferens） feeding on the Bt rice with a fused CrylAb＋CrylAc gene （cv. , Bt Huahui1 ） and its parent line of nontransgenic rice （ cv. , Minghui63）. The results indicate that no significant differences were found in the number of egg masses per 100 plants for C. suppressalis or S. inferens occurring on Bt rice in contrast to nontransgenic rice. In addition, no significant differences in egg mass densities between C. suppressalis and S. inferens were found when fed on nontransgenic rice, whereasas a significant increase in egg mass were observed for C. suppressalis relative to S. inferens fed on Bt rice. Compared with nontransgenic rice, Bt rice significantly reduced the population density （ i. e. , individuals per 100 plants; 84. 9%-100% ） of C. suppressalis larvae, while the transgenicity did not significantly influence the population abundance for S. inferens. Moreover, C. suppressalis had significantly higher larval abundances than S. inferens when they fed on nontransgenic rice, while no signifi

关 键 词：BT水稻 靶标/次靶标抗性 二化螟 大螟 种群发生 生态风险 

分 类 号：S435.112.1[农业科学—农业昆虫与害虫防治]