沙冬青A20/AN1锌指蛋白基因序列及表达分析  

Sequence analysis and expression pattern of AmSTZF encoding an A20/AN1 zinc finger protein in Ammopiptanthus mongolicus.

作　　者：史军娜[1] 刘美芹[1] 刘杰[1] 陈玉珍[1] 卢存福[1] 

机构地区：[1]北京林业大学林木育种国家工程实验室教育部林木花卉遗传育种重点实验室

出　　处：《北京林业大学学报》2012年第2期103-108,共6页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(30700559;30671476)

摘　　要：A20/AN1锌指蛋白普遍存在于真核生物中,在胁迫响应引起的信号传导过程中发挥着重要作用。本文利用生物信息学和RT-PCR方法从低温干旱胁迫的沙冬青叶片组织中分离锌指蛋白基因AmSTZF,该基因编码一条含172个氨基酸残基的多肽,含A20和AN1两个锌指蛋白保守结构域,属A20/AN1锌指蛋白。生物信息学分析显示该蛋白定位于细胞质中,具有磷酸化位点,参与细胞转录调控。RT-PCR分析表明:低温和干旱胁迫处理的沙冬青叶片中,AmSTZF的表达增强,初步推测该基因与沙冬青低温、干旱胁迫响应相关。A20/AN1 zinc finger protein widely exists in eukaryote and plays a central role in signal transduction pathway involved in stress response. We cloned zinc finger gene AmSTZF from cold- and drought-treated Ammopiptanthus mongolicus leaves by bio-informatics and RT-PCR. The cDNA of AmSTZF encodes a polypeptide with 172 amino acids,which contains A20 and AN1 conserved domains and belongs to A20/ AN1 zinc finger protein. Bio-informatics predicts that AmSTZF has phosphorylation sites, exists in cytoplasm and is involved in the regulation of gene transcription. RT-PCR analysis shows that the expression of AmSTZF is up-regulated under cold- and drought-stresses and that AmSTZF is related to the response to drought and chilling stresses.

关 键 词：沙冬青 A20/AN1锌指蛋白 序列分析 表达分析 

分 类 号：S759.95[农业科学—森林经理学]