参芎葡萄糖注射液对缺血再灌注损伤心肌的保护作用  

The cardio-protection of Salvia miltiorrhiza Ligustrazine Hydrochloride and Glucose Injection on myocardial ischemia-reperfusion injury in mice

作　　者：李达文[1] 龚惠[1] 叶勇[1] 路静[2] 蒋国良[1] 贾剑国[3] 吴超能[3] 张国平[1] 邹云增[1,3] 

机构地区：[1]复旦大学生物医学研究院,上海200032 [2]同济大学东方转化医学研究中心,上海200120 [3]复旦大学附属中山医院上海市心血管病研究所,上海200032

出　　处：《中国分子心脏病学杂志》2012年第1期51-56,共6页Molecular Cardiology of China

基　　金：国家自然基金重点项目(30930043);国家自然基金青年基金项目(81000041);上海市重点专项课题(08dj1400504)

摘　　要：目的探讨参芎葡萄糖注射液对小鼠急性心肌缺血再灌注(I/R)损伤的保护作用及可能的分子机制。方法建立急性心肌缺血再灌注(Ischemia-Reperfusion,I/R)损伤的小鼠模型,将野生型小鼠(C57BL/6)随机分为3组,假手术组(Sham),缺血再灌注+生理盐水组(Vehicle),缺血再灌注+参芎葡萄糖注射液预处理组(20mL/kg,SL),术前2小时给予腹腔注射生理盐水或参芎葡萄糖注射液,于缺血再灌注后不同时间点,通过心脏超声观察各组小鼠心功能的变化,心肌组织TTC染色确定心梗面积大小,脱氧核糖核苷酸末端转移酶法(TUNEL)检测凋亡心肌,蛋白免疫印迹法(Western Blot)及酶联免疫吸附法(ELISA)分别检测心肌组织以及血清炎症因子肿瘤坏死因子(TNF-α)表达水平变化。结果 I/R手术48小时后,与假手术组相比,左室射血分数(LVEF)明显降低(P<0.01)。参芎葡萄糖注射液预处理明显改善I/R组的LVEF,并降低了心肌梗死面积,抑制I/R引起的心肌细胞凋亡和组织炎症反应。进一步的研究表明,参芎葡萄糖注射液预处理明显抑制了I/R后心肌组织以及血清中TNF-α表达水平升高(P<0.01)。结论参芎葡萄糖注射液预处理通过抑制TNF-α表达水平发挥对缺血再灌注(I/R)损伤心肌的保护作用。Objective To investigate the cardio-protective effect of Salvia miltiorrhiza Ligustrazine Hydrochloride and Glucose injection （SL） induced by ischemic pre-conditioning （IPC） following myocardial ischemia-reperfusion （I/R） injury in mice. Methods I/R injury model was obtained by ligating the proximal left anterior descending coronary artery 30 min followed by different hours reperfusion. Wild-type C57BL/6 mice were randomly divided into 3 groups and separately subjected to IPC treatments： Sham control group; I/R＋Vehicle control group; I/R＋SL group （20 mL/ kg）; The mice were treated with the SL by peritoneal injection 2 hours before the surgery. Cardiac function was detected by echocardiography, TTC staining was performed to evaluate the area of myocardial infarction, The number of apoptotic cardiomyoeyte was detected by TUNEL assay, TNF-α levels in serum and myocardium was measured by ELISA and Western Blot, respectively. Results Cardiac function of mice in SL group were greatly improved at 48 hours after I/R surgery （P〈0.01）. Further research indicated that SL pretreatment significantly reduced infarct size, inhibited apoptosis, attenuated inflammation response in myocardium, and decreased TNF-α level in serum and myocardium, compared with the vehicle group （P〈0.01）. Conclusion ＂Salvia miltiorrhiza Ligustrazine Hydrochloride and Glucose injection＂ exerts cardio-protective effects on ischemia-reperfusion injury by inhibiting TNF-α level.

关 键 词：参芎葡萄糖注射液 心肌缺血再灌注损伤 炎症反应 细胞凋亡 

分 类 号：R965[医药卫生—药理学]