青年母猪不同生殖器官神经营养因子-4/5的表达研究  

Expression of Neurotrophin-4/5 in the Reproductive Organs of the Prepubertal Gilts

作　　者：张伟[1] 易康乐[2] 周虚[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]湖南省畜牧兽医研究所,湖南长沙410000

出　　处：《中国畜牧兽医》2012年第3期41-46,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家重点基础研究发展计划(973)项目(2011CB101003)

摘　　要：本研究旨在探讨神经营养因子-4/5(neuroteophin-4/5,NT-4/5)在青年母猪卵泡期与黄体期卵巢、输卵管及子宫3种生殖器官中表达情况。采用实时荧光定量RT-PCR方法与Western blotting技术对青年母猪卵泡期与黄体期卵巢、输卵管及子宫中的NT-4/5mRNA与蛋白质表达进行检测。结果表明,NT-4/5mRNA与蛋白质在卵泡期与黄体期卵巢、输卵管及子宫中均有表达,且卵泡期卵巢、子宫NT-4/5mRNA表达水平均显著高于黄体期(P<0.05),而卵泡期输卵管NT-4/5mR-NA表达水平显著低于黄体期(P<0.05)。试验结果为进一步研究NT-4/5参与青年母猪生殖道生理功能的调节奠定基础。The expression of neurotrophin-4/5（NT-4/5） mRNA and protein in ovary,oviduct and uterus during the follicle and luteal phase of the pubertal gilts were investigated by quantitative real-time PCR and Western blotting.The results showed that the mRNA and protein of NT-4/5 were present in ovary,oviduct and uterus during the follicle and luteal phase.In addition,the expression amount of NT-4/5 mRNA in ovary,uterus during the follicle stage were significantly higher than the luteal phase（P0.05）,however,the expression level of NT-4/5 mRNA in oviduct during the follicle phase was significantly lower than the luteal phase（P0.05）.In collection,these results strongly suggest that local production of the NT-4/5 may particulate in the regulation of reproductive functions in the reproductive functions in the reproductive tract of the prepubertal gilts.

关 键 词：生殖器官 NT-4/5 青年母猪 

分 类 号：Q421[生物学—神经生物学]