江苏地方山羊品种H-FABP基因的SNPs检测及群体遗传学分析  

SNPs Detection and Genetic Analysis on H-FABP Gene of Jiangsu Local Goat Breeds

作　　者：王兰萍[1] 耿荣庆[1] 冀德君[2] 常洪[2] 

机构地区：[1]盐城师范学院生命科学与技术学院,江苏盐城224051 [2]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《中国畜牧兽医》2012年第3期159-162,共4页China Animal Husbandry & Veterinary Medicine

基　　金：江苏省高校自然科学基础研究项目(09KJD230001)

摘　　要：采用PCR-SSCP和DNA测序技术对2个江苏地方山羊品种的心脏型脂肪酸结合蛋白(heart fatty acid binding pro-tein,H-FABP)基因进行单核苷酸多态性(SNP)检测和群体遗传学分析。结果表明,在所扩增的片段中,仅在H-FABP基因外显子2的第132位检测到G/C碱基突变,形成GG和GC基因型,2个山羊群体均是纯合GG基因型占主导优势,且该位点处于Hardy-Weinberg平衡状态;黄淮山羊群体的杂合度、有效等位基因数和多态信息含量指标均高于长江三角洲白山羊群体,表明其群体内的遗传变异程度相对较大、遗传多样性也更为丰富。PCR-SSCP and DNA sequencing approaches were applied to assess the single nucleotide polymorphisms（SNPs） and analyze the population genetics of heart fatty acid binding protein（H-FABP） gene of two goat breeds in Jiangsu province.The results showed that only the 132 G/C mutation was detected at the H-FABP gene exon 2 of the amplified fragments,with the formation of GG and GC genotypes.Homozygous GG was the dominant genotype of two goat groups,and the locus was at Hardy-Weinberg equilibrium.Heterozygosity,effective number of alleles and polymorphism information content of Huanghuai goat population was higher than the Yangtze River Delta white goat populations,indicating a relatively large degree of genetic variation and more abundant genetic diversity within population.

关 键 词：山羊 H-FABP基因 SNPS PCR-SSCP 

分 类 号：Q31[生物学—遗传学]