清远麻鸡禽白血病病理组织学观察和PCR诊断  

Histopathological Observation and PCR Diagnosis of Avian Leukosis in Qingyuan MA Chicken

作　　者：黄兴国[1] 王政富[1] 高明超 蔡丽丽[1] 王俊峰[1] 黄淑坚[1] 

机构地区：[1]佛山科学技术学院,广东佛山528231 [2]广东天农食品有限公司,广东清远513100

出　　处：《中国畜牧兽医》2012年第3期197-201,共5页China Animal Husbandry & Veterinary Medicine

基　　金：广东省科技厅农业攻关项目(2009B020418003)

摘　　要：本试验选择临床症状明显、经禽白血病抗原检测为阳性的15只祖代清远麻鸡,采集肝脏、脾脏、肾脏等器官进行病理组织学观察和PCR诊断。结果显示,从病理组织切片中观察到肝脏、肾脏、脾脏、骨髓、腺胃、心脏、肺脏、胰腺和卵巢等组织中有大量的淋巴母细胞呈灶状增生或散在分布,以及少量的成髓细胞增生。禽白血病病毒主要侵害祖代清远麻鸡机体的内脏器官和骨髓,对大脑等神经组织损害较轻;PCR扩增结果表明,所采集的15份样品均能扩增出545bp左右的特异性片段,证明15份组织样品中均存在J亚型禽白血病病毒。15 Qingyuan MA chickens which appeared obviously clinical symptom and were tested to be avian leukosis positive were selected. The tumor organs, such as liver, spleen, kidney and so on, were carried on histopathology and PCR diagnosis. The pathology tissue slices from organs of liver, kidney, spleen, marrow, gland stomach, heart, lung, pancreatic gland ovary and so on, were observed, results showed that the proliferation of massive lymphoblasts or scatter and of small lymphomyelocytes were observed. The PCR identification indicated that all 15 samples can amplify a fragment about a length of 545 bp, which consisted with the expected size. The results suggested that all 15 isolates were the ALV-J avian Leukosis viruses.

关 键 词：清远麻鸡 禽白血病 病理组织学 PCR 

分 类 号：S854[农业科学—临床兽医学]