松弛素在大鼠矽肺形成过程中肺组织的表达及作用  

Expression and Potential Role of Relaxin in Lung Tissue of Rats Developing Silicosis

作　　者：李小峰[1] 廖静[1] 鲁文清[1] 刘爱林[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系,环境与健康教育部重点实验室,湖北武汉430030

出　　处：《环境与职业医学》2012年第3期132-135,共4页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金青年科学基金项目(编号:30800901)

摘　　要：[目的]探讨松弛素(relaxin)在石英尘诱导矽肺形成中的潜在作用。[方法]建立矽肺体内动物模型和体外细胞模型,在动物模型中应用免疫组织化学法和实时荧光定量PCR分析肺组织松弛素蛋白及基因表达,在细胞模型中通过酶联免疫法测人胚肺成纤维细胞(HFL-Ⅰ)Ⅰ型胶原分泌量。[结果]松弛素蛋白在大鼠正常肺组织中弱表达,主要定位于Ⅰ型肺泡细胞。大鼠染石英尘(DQ12)后肺组织松弛素蛋白和mRNA表达呈现先上升后下降的趋势,主要定位于Ⅰ型肺泡细胞和巨噬细胞;与对照组相比,染石英尘后第7天松弛素蛋白水平达到最高(P<0.05),第14天时无明显差异(P>0.05),第28天时明显减少(P<0.05)。与DQ12组相比,松弛素处理组中HFL-Ⅰ细胞Ⅰ型胶原的生成明显减少(P<0.05)。[结论]松弛素可以抑制石英尘诱导的肺成纤维细胞胶原合成,可能对石英尘诱导矽肺发生有影响。[ Objective ] To investigate the potential role of relaxin in the development of silica-induced silicosis.[ Methods ] Both in vitro and in vivo models of silicosis were established. Relaxin gene and protein expression in rat lung tissues were determined by real-time quantitative polymerase chain reaction （PCR） and immunohistochemical staining, respectively. Type I collagen in human fetal tung fibroblasts （HFL-I） supernatants was measured by enzyme-linked immunosorbent assay （ELISA）. [ Results ] Slight expression of relaxin was observed in the normal control rat lung tissues and the signal intensities were primarily located in pulmonary alveolar type I cells. The relaxin protein and gene expression in the lungs of silica-treated （Dorentrup quartz, DQ12） rats showed a tendency of increasing first and then decreasing and was primarily located in pulmonary alveolar macrophages and type I cells. Compared with the controls, the relaxin protein expression reached maximum at 7 d （P 〈 0.05）, then went down at 14 d （P 〉 0.05）, and decreased markedly at 28 d （P 〈 0.05）. The levels of type I collagen in HFL-I cells in the relaxin group was significantly lower than that in the DQ12 group （P〈0.05）. [Conclusion] Relaxin can inhabit the formation of silica-induced type I collagen in lung fibroblasts, which may affect the development of silica-induced silicosis.

关 键 词：矽肺 肺纤维化 松弛素 

分 类 号：R114[医药卫生—卫生毒理学]