中国美利奴羊DLX3基因3′UTR的多态性及其与羊毛品质性状的关联分析  

Polymorphism in 3′UTR of DLX3 Gene and Its Association with Wool Quality Traits in Chinese Merino Sheep

作　　者：荣恩光[1] 王志鹏[1] 张志威[1] 杨华[2] 李辉[1] 王宁[1] 

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030 [2]新疆农垦科学院畜牧兽医研究所,石河子832000

出　　处：《畜牧兽医学报》2012年第3期358-367,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家转基因专项课题(2008ZX08009-002;2009ZX08009-160B)

摘　　要：为探讨DLX3基因的多态性与绵羊羊毛品质及生长性状的关系,本研究以5个品系的中国美利奴羊(新疆军垦型)为试验材料,采用PCR-RFLP方法开展了DLX3基因3′UTR区4个SNPs的多态性检测。通过卡方检验分析了4个SNPs在各品系绵羊中的等位基因频率,采用连锁不平衡分析构建了这4个SNPs的单倍型,最后将单位点和单倍型分别与绵羊羊毛品质和生长性状进行关联分析。结果表明:4个SNPs在5个品系间的等位基因频率均存在极显著差异(P<0.01);超细毛绵羊品系与其他4个品系间的基因型分布均存在极显著差异(P<0.01),2个多胎品系与其他3个品系间的基因型分布同样存在极显著差异(P<0.01);相关分析显示,这4个SNPs及其单倍型均对羊毛卷曲度有显著影响(P<0.05),而对其他羊毛性状及生长性状无显著影响(P>0.05)。由此可见,中国美利奴羊(新疆军垦型)DLX3基因3′UTR区的多态性与绵羊毛发卷曲度性状显著相关,可以尝试使用这些SNPs开展高品质细毛羊的分子标记辅助选择。This experiment was designed to study the effects of polymorphism of DLX3 gene on wool quality and growth traits in sheep. Five strains of Chinese Merino （Xinjiang Junken Type） sheep were used in the present study. The polymorphism in 3＇ untranslated region （UTR） of DLX3 gene was detected and identified using DNA sequencing, and SNP genotyping was per- formed by PCR-RFLP analysis. Allele frequencies were analyzed by Chi-square test. Haplotypes were constructed by linkage disequilibrium analysis. The association between the SNPs and hap lotypes and wool quality and growth traits were assessed by JMP4.0 software, respectively. The result showed that the allele frequencies for these four SNPs were significantly different among the five Chinese sheep strains （P〈0.01）. The genotype distribution in super fine wool strain were different from that in the other four strains, and the genotype distribution in two prolificacy strains were significantly different from that in other three no-prolificacy strains （P〈0. 01）; Association analysis displayed that the four SNPs and their haplotypes had consistently significant effect on wool curvature （P〈0.05）, but no significant effect on other traits （P〈0.05）. Theresult indicate that polymorphisms of 3＇UTR of curvature in Chinese Merino （Xinjiang Junken molecular MAS for wool quality traits. DLX3 gene are significantly correlated with wool Type） sheep, and these SNPs can be used in

关 键 词：中国美利奴羊(新疆军垦型) DLX3基因 SNPS 3′UTR 

分 类 号：S826[农业科学—畜牧学] Q343.15[农业科学—畜牧兽医]