副猪嗜血杆菌外膜蛋白表型分析  

Analysis of Outer Membrane Proteins Phenotype of Haemophilus parasuis

作　　者：朱必凤[1] 杨旭夫[1] 彭凌[1] 韦昭玉[2] 

机构地区：[1]韶关学院英东生命科学学院,韶关512005 [2]南昌大学生命科学学院,南昌330047

出　　处：《畜牧兽医学报》2012年第3期496-502,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：香港霍英东铭源基金(HK314-14591);广东省农业攻关项目(2004B20201019);韶关市科技创新项目(2010140473)

摘　　要：为揭示副猪嗜血杆菌外膜蛋白与毒力的关系,采用SDS-PAGE测定了82个副猪嗜血杆菌分离株细胞外膜蛋白(OMP),比较了不同临床背景分离株的OMP表型差异,根据外膜蛋白的电泳迁移率Rf值和蛋白含量对OMP与毒力菌株的相关性和PAGE分型进行了聚类分析。图谱表型分析结果表明,82个分离株分成以相对分子质量36～40ku为特征的Ⅰ型和以42～45ku为特征的Ⅱ型2种类型,约34%患病猪分离株属于PAGEⅠ型,只有8.7%健康猪分离株属于PAGEⅠ型。Rf值聚类分析结果显示外膜蛋白分为3种类型,PAGEⅠ、PAGEⅡ和PAGEⅢ型。图谱蛋白含量聚类分析显示5个PAGE型。结果提示,36～40ku蛋白与菌株的毒力相关。The aim of this study was to explose relatioship between outer menbrane protein and virulence of Haernophilus parasuis. The outer membrane proteins （OMP） of 82 isolates of Hae- rnophilus parasuis were determined by SDS-PAGE. We compared the diversity of the OMP phe- notype of isolates from diffent clinical background to explore the associativity between OMP and virulence pathogen, and analyzed relationship between the OMP and virulen isolates by cluster analysis based on RI of electrophoresis and contents of OMP. The results of analysis of OMP phenotye showed that isolates were divided into two major PAGE type groups as differece of Rf. PAGE I was characterized from 36 to 40 kD OMP and PAGE Ⅱ was characterized 42-45 kD OMP, and included about 34% isolates recovered from disease pigs and 8. 7% isolates recovered from healthy pigs belong to PAGE I; PAGE II was characterized by absence of the 36-40 kD proteins. The result of cluster as Rf indicated that isolates were divided into three types. PAGE I , PAGE Ⅱ and PAGEⅢ. Analysis of cluster as contents of OMP obtained five classsifications of isolates. It is prompted that the 36-40 kD OMP was correlated with virulence.

关 键 词：副猪嗜血杆菌 外膜蛋白 毒力菌株 聚类分析 PAGE分型 

分 类 号：S852.61[农业科学—基础兽医学]