2型糖尿病患者外周血单个核细胞组蛋白乙酰化研究  

Histone acetylation in peripheral blood mononuclear cells of type 2 diabetic patients

作　　者：侯粲[1] 赵明[2] 李霞[1] 李一君[1] 蔺怡[1] 陆前进[2] 周智广[1] 

机构地区：[1]中南大学湘雅二医院内分泌科,中南大学糖尿病中心,中南大学代谢内分泌研究所,糖尿病免疫学教育部重点实验室,长沙410011 [2]中南大学湘雅二医院皮肤／性病科,医学表观基因组学湖南省重点实验室

出　　处：《中华内分泌代谢杂志》2012年第3期183-185,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：国家重点基础研究发展计划（973计划）项目（2009CB825605）

摘　　要：目的本研究探讨了2型糖尿病患者外周血单个核细胞（PBMC）组蛋白乙酰化修饰状态。方法收集12例2型糖尿病患者及12名健康对照者的PBMC，H3／H4乙酰化检测试剂盒提取组蛋白，检测H3／H4乙酰化水平。实时定量PCR检测2型糖尿病PBMC组蛋白修饰基因mRNA表达水平。结果与健康对照组比较，2型糖尿病组PBMC组蛋白H3／H4乙酰化水平升高（H3：0．134±0．035对0．181±0．023，P〈0．05；H4：0．266±0．070对0．324±0．062，P〈0．01）。2型糖尿病组PBMC的p300、CREBBP、HDAC2、HDAC7、SIRT1基因表达分别是健康对照组的3．11±0．38、2．78±0．45、3．55±0．30、0．77±O．37、0．40＋0．25倍。结论2型糖尿病患者外周血PBMC组蛋白乙酰化修饰呈现异常。Objective To study the histone acetylation patterns in peripheral blood mononuclear cells （PBMC） from type 2 diabetic patients. Methods PBMC from 12 type 2 diabetic patients and 12 healthy control subjects were collected. The global histone H3/H4 acetylation was determined by assay kit. The differential expression of the histone acetyhransferases and histone deacetylases were measured by real time PCR. Results Increased H3/H4 acetylation was observed in PBMC of type 2 diabetic patients compared with control subjects （ H3 ： 0. 134±0. 035 vs O. 181±0. 023 ,P〈0. 05;H4：0. 266±0. 070 vs O. 324±0. 062 ,P〈0. 01 ）. The mRNA levels of p300, CREBBP, HDAC2, HDAC7, and SIRT1 in patients＇ PBMC compared with healthy control subjects were 3.11 ± 0. 38,2. 78±0.45,3.55±0. 30,0.77±0. 37, and 0.40±0. 25 folds, respectively. Conclusion Histone acetylation appears abnormal in PBMC of type 2 diabetic patients.

关 键 词：糖尿病 2型 外周血单个核细胞 组蛋白乙酰化 

分 类 号：R587.1[医药卫生—内分泌]