毛细管电泳电化学发光法同时测定盐酸格拉司琼和盐酸阿扎司琼  

Determination of granisetron hydrochloride and azasetron hydrochloride by capillary electrophoresis coupled with electrochemical luminescence method

作　　者：陈鑫 木合塔尔.吐尔洪 宋海林[3] 李明月[3] 

机构地区：[1]陕西省汉中市405医院,汉中723312 [2]喀什师范学院,喀什844007 [3]西安交通大学分析研究所,西安710061

出　　处：《西北药学杂志》2012年第2期113-116,共4页Northwest Pharmaceutical Journal

基　　金：新疆维吾尔自治区高校科研计划重点项目(编号:XJEDU2008145)

摘　　要：目的基于叔胺结构的5-HT3拮抗剂对三联吡啶钌溶液的电化学发光信号具有显著增敏作用,建立应用毛细管电泳-电化学发光方法同时检测分析2种5-HT3拮抗剂盐酸格拉司琼、盐酸阿扎司琼的方法。方法采用正交实验设计,对毛细管电泳分离条件和电化学发光检测条件进行了优化。结果在优化的实验条件下,可在200s内实现盐酸格拉司琼和盐酸阿扎司琼的分离和检测,其质量浓度线性范围为3.0×10-7～1.0×10-4 g.mL-1;线性方程分别为I=48.990C+47.365(r=0.995 9)和I=52.605C+105.70(r=0.991 8);检出限分别为5.6(S/N=3)和3.9ng.mL-1(S/N=3);平均回收率分别为98.9%和101.3%,其RSD分别为0.74%和1.29%。结论该法灵敏简便,具有较强专属性,可同时检测盐酸格拉司琼和盐酸阿扎司琼。Objective Since the significant sensitizing effect on electrochemical luminescence （ELC） intensity of the structure of terti- ary amine on the 5-HTa antagonist ruthenium terpyridine solution was achieved,a method for determination of granisetron hydro- chloride and azasetron hydrochloride was established using capillary electrophoresis （CE） and electrochemical luminescence（ECL） detection. Methods Based on orthogonal test design,parameters affecting separation of capillary CE and ECL detection were opti- mized respectively. Results Under the optimized experiment conditions, granisetron hydrochloride and azasetron hydrochloride could be separated and detected within 200 s in this method. The ECL intensity was linear with granisetron hydrochloride and azasetron hydrochloride concentration in the range of 3. 0 × 10-7-1.0 × 10.4 g · mL-1（ n= 5 ）, and their linear equations were 1=48. 990Cq-47. 365 （r=0. 995 9） and I=52. 605C＋105.70 （r=0. 991 8）. The detection limits were 5.6[S/N=3） and 3.9 ng · mL-1 （ S/N= 3） ;and the average recoveries were 98.9 % and 101.3 %, respectively, with RSD 0.74 % and 1.29 %, respectively. Conclusion The method is sensitive and convenient,and can be applied to detect granisetron hydrochloride and azasetron hydrochlo- ride injection successfully.

关 键 词：毛细管电泳 电化学发光 叔胺类药物 5-HT3拮抗剂 

分 类 号：R927.2[医药卫生—药学]