苍术抗流感病毒有效成分的筛选  

Effective Component Selection of Atraotydin Extract against Influenza Virus

作　　者：石书江[1] 秦臻[2] 孔松芝[2] 赖小平[2] 苏子仁[2] 曾惠芳[1] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]广州中医药大学中药学院,广东广州510006

出　　处：《时珍国医国药》2012年第3期565-566,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.U0732004);国家科技重大专项(No.2009ZX09103-376)

摘　　要：目的从苍术中筛选抗流感病毒的有效成分。方法采用水提、超临界二氧化碳流体(SFE-CO2)提取,并利用硅胶柱层析法进行成分分离,得到苍术的各种提取物和有效成分单体,再用流感病毒甲型H3N2、H5N1(禽流感病毒)和乙型流感病毒进行狗肾传代细胞株(MDCK)组织培养筛选。结果含苍术酮的苍术SFE-CO2提取物及苍术酮,对流感病毒有杀灭作用;而苍术素、苍术的水提液对流感病毒无抑制作用。结论苍术酮是苍术中抗流感病毒的有效成分。Objective To screen the active components against anti influenza virus in Atraotydin. Methods The extracts were separated by supercritical CO2 fluid extraction（SFE-CO2）,aqueous extraction and silica column chromatography.Madin-Darby Canine Kidney（MDCK） cell tissue culture was used to screen the effective ingredient with fluenza A virus and influenza B virus. Results Atractylon and the SFE-CO2 Atracylodes Lancea Rhizoma could kill influenza virus;Atractylodin and the water extract from Rhizoma Atractylodis had no effect on influenza virus. Conclusion Atractylon is effective ingredient for anti influenza virus in Atraotydin.

关 键 词：苍术 苍术酮 流感病毒 筛选 

分 类 号：R285.5[医药卫生—中药学]