不同产地安息香中肉桂酸的含量比较  

The Content Comparison of Cinnamic Acid in Benzoinum from Different Habitats

作　　者：黄立华[1] 夏厚林[1] 王建[1] 彭颖[1] 周颖[1] 范成杰[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室,四川成都611137

出　　处：《时珍国医国药》2012年第3期596-597,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家"973"重点基础研究发展规划项目(No.2007CB512606)

摘　　要：目的建立安息香中肉桂酸含量测定方法,并比较不同产地安息香中肉桂酸的含量差异。方法使用Agilent TC-C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水-冰醋酸(40∶60∶0.6),流速1.0 ml/min,柱温25℃,检测波长为270 nm。结果肉桂酸在0.110 5～1.105μg范围内与峰面积呈良好的线性关系;平均回收率为100.81%,RSD为2.89%。不同产地药材中肉桂酸的含量范围为0.047%～0.103%。结论中国、苏门答腊、泰国3个产地安息香中肉桂酸的含量没有显著性差异。Objective To establish a method for determination of the content of cinnamic acid in Benzoinum and compare the content from different habitats. Methods The Agilent TC-C18 analytical column（250 mm ×4.6 mm,5 μm） was used with Methanol-water-acetic acid（40： 60： 0.6）as the mobile phase at a flow rate of 1.0 ml·min-1.The column temperature was at 25℃ and the detection wavelength was set at 270 nm. Results There was a good linear relationship between content and peak area when the content was within the range of 0.110 5~1.105 μg.The average recovery was 100.81%,and RSD was 2.89%.The content of cinnamic acid in Benzoinum from different habitats ranged from 0.047% to 0.103%. Conclusion There has no significant difference in content of cinnamic acid among China,Sumatra and Thailand.

关 键 词：不同产地 安息香 肉桂酸 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]