水通道蛋白1、4、5在外燥小鼠肺表达的意义  

Significance of Aquaporin1,4,5 Expression in Lung Tissue of Mice with External Dryness

作　　者：丁建中[1] 张六通[2] 李军川[1] 殷涛[2] 张燕翔[1] 

机构地区：[1]长江大学医学院,湖北荆州434023 [2]湖北中医药大学,湖北武汉430065

出　　处：《时珍国医国药》2012年第3期764-765,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30672572);湖北省自然科学基金项目(No.2009CDB289)

摘　　要：目的观察外燥对小鼠肺组织水通道蛋白1、4、5(AQP1、4、5)表达的影响。方法 81只SPF级昆明种小鼠随机分为常温常湿组、温燥组、凉燥组,每组27只,按文献造模处理后第6天、第12天、第18天进行肺组织病理观察、AQP1、4、5阳性表达率与二棕榈酰卵磷脂(DPPC)含量检测。结果温燥组、凉燥组第6～18天肺泡瘀血、炎性细胞浸润伴肺气肿。与常温常湿组比较,温燥组、凉燥组第6～18天DPPC与AQP1、5表达阳性率持续下降(P<0.01)。结论外燥伤肺,可致Ⅱ型肺泡细胞分泌DPPC持续减弱而影响调节肺泡张力;同期肺AQP1、5表达率下降可致肺泡液细胞内外水液转运失衡而与病变相关,提出AQP1、5与DPPC可以作为研究外燥伤肺、耗津病机的特异性指标之一。Objective To investigate the effects of exernal dryness on the express of Aquaporin 1,4,5（AQP1,4,5） in lung tissue of mice. Methods 81 Kunming mice（SPF） were randomly divided into three groups：group A（normal mice）,group B（Warm-dryness）and group C（Cool-dryness）,27 mice in each group.Pathological change,express of AQP1,4,5 and Dipalmitoyl Phosphatidyl Choline（DPPC）were detected on day 6,12 and18. Results In day 6-18 congestion,infilaration of inflammative cell and emphysema of pulmonary alveolus in group B and group C were observed.Compared with group A,DPPC level and the expression of AQP1,4,5 were decreased significantly in group B,C（P0.01）. Conclusion The reduction of DPPC from TypeⅡalveolar epithelial cells and the positive express of AQP1,5 are related with injury of lungs and ＂lung-fluid trasporting＂,which may be one of the specific marks to research the external dryness mechanisms.

关 键 词：外燥 肺 水通道蛋白 二棕榈酰卵磷脂 小鼠 

分 类 号：R284.2[医药卫生—中药学]