不同基因型别的阴道白假丝酵母菌黏附性的研究  

In vitro adherence activity of different genotypes of Candida albicans from patients with vulvovaginal candidiasis

作　　者：张旭[1] 刘魏[2] 刘朝晖[1] 

机构地区：[1]北京大学第一医院妇产科,北京100034 [2]暨南大学附属第一医院妇产科,广州510630

出　　处：《现代妇产科进展》2012年第3期205-207,共3页Progress in Obstetrics and Gynecology

摘　　要：目的:研究不同基因型别的阴道白假丝酵母菌黏附性的差异。方法:为阴道白假丝酵母菌进行25S rDNA基因分型,随机选取A、B、C型菌株各20株测定黏附率。结果:pH环境相同时(pH7.0或pH4.0),孢子相菌株A型黏附率显著高于B型(P<0.05)和C型(P<0.001),B型显著高于C型(P<0.01),而菌丝相菌株各基因型别黏附率无统计学差异;孢子相菌株不含内含子组(A型)黏附率显著高于含内含子组(B型和C型)(P<0.001),而菌丝相菌株两组间黏附率无统计学差异。结论:阴道白假丝酵母菌25S rDNA可转座Ⅰ型内含子与其黏附性密切相关。Objective： To investigate the in vitro adherence to VEC（vaginal epithelial cell）of different genotypes of candida albicans from patients with vulvovaginal candidiasis.Methods：Isolates of Candida albicans were genotyped by polymerase chain reaction（PCR）of the 25S rDNA.Sixty isolates（20 strains for each genotype,respectively）were randomly selected to detect their adherence activity.Results：In the same environmental pH（pH 7.0 or pH 4.0）,the yeast-phase organisms with genotype A had stronger adherence than both genotype B（P0.05）and genotype C（P0.001）;genotype B had stronger adherence than genotype C（P0.01）.The adherence activity of the yeast-phase isloates with the intronless group（genotype A）was higher than the intron-containing group（genotype BC,P0.001）.Conclusion：The presence or absence of the transposable group I intron in the 25S rDNA gene may be important in determining the adherence activity of Candida albicans.

关 键 词：外阴阴道假丝酵母菌病 白假丝酵母菌 基因分型 二相性 黏附 

分 类 号：R711.31[医药卫生—妇产科学]