胰岛素样生长因子1在新生大鼠短暂脑缺血损伤修复中的关键作用  

Insulin-like growth factor 1 playing a pivotal role on recovery of transient forebrain ischemia damage in neonatal rats

作　　者：曾文钦[1] 杨春[1] 周辛[1] 段富华[1] 刘玉峰[1] 戴景兴[1] 原林[1] 

机构地区：[1]南方医科大学基础医学院人体解剖学教研室,广州510515

出　　处：《解剖学报》2012年第2期145-149,共5页Acta Anatomica Sinica

基　　金：国家重点基础研究计划(973计划)资助项目(2007CB512705);国家自然科学基金青年基金资助项目(30801464);广东省高新技术产业化项目-工业攻关"自体筋膜组织系统的研发(2011B010500004)"

摘　　要：目的 7日龄新生大鼠短暂脑缺血诱导侧脑室室管膜下区(SVZ)神经干细胞增殖,观察胰岛素样生长因子1(IGF-1)对神经干细胞增殖的影响。方法 7日龄新生SD大鼠64只,随机分为缺血组(n=24)、假手术组(n=24)、缺血阻断剂组(n=8)和缺血生理盐水组(n=8)。利用免疫组织化学方法观察缺血组和假手术组在缺血后1、4、7d SVZ新生细胞数量和IGF-1表达的变化,并观察缺血阻断剂组和缺血生理盐水组在IGF-1受体阻断剂(JB1)阻断7d后SVZ新生细胞数量和IGF-1表达的变化。结果与同时间点假手术组比较,缺血后1、4、7d的SVZ新生细胞和IGF-1阳性细胞数量均显著增加,差异有统计学意义(P<0.05);使用JB1后,缺血阻断剂组IGF-1的表达被阻断,IGF-1阳性细胞缺如,而缺血生理盐水组IGF-1表达正常;使用JB1后,缺血阻断剂组SVZ新生细胞数量显著减少,与缺血生理盐水组相比,差异有统计学意义(P<0.05)。结论新生大鼠缺血再灌注损伤上调了IGF-1的表达,IGF-1表达增加促使SVZ神经干细胞增殖;使用JB1后,IGF-1的表达被阻断,神经干细胞增殖也显著减少,提示IGF-1在脑缺血损伤修复中起关键作用。Objective To study the impact of insulin-like growth factor 1 （IGF-1） on the neural stem cell proliferation of the subventricular zone （SVZ） induced by transient forebrain ischemia in postnatal day 7 rats. Methods A total of 64 postnatal day 7 Sparuge-Dawley rats were randomly divided into ischemia group （ n = 24） , sham group（ n = 24） , ischemia plus antagonist group （ n = 8） and ischemia plus saline group （ n = 8）. The cell proliferation and IGF-1 in the SVZ of the ischemia group and the sham group were observed 1, 4 or 7 days after ischemia with immunohistochemical staining. The cell proliferation and IGF-1 in the SVZ of the ischemia plus antagonist group and the ischemia plus saline group were detected after 7 days of continuous treatment with JB1 or without JB1 treatment. Results The number of BrdU ＋ cells and IGF-1 ＋ cells of the ischemia group were significantly increased in the SVZ 1, 4, and 7 days after ischemia compared with that of the sham group （P 〈 0. 05）. After the administration of JBI, the IGF-1 expression was blocked. The IGF-1＋ cells were absent in ischemia plus antagonist group, while the IGF-1 expressed normally in ischemia plus saline group. The number of BrdU ＋cells in the ischemia plus antagonist was sharply decreased compared with the ischemia plus saline group （ P 〈 0.05 ） in the SVZ 7 days after ischemia. Conclusion Ischemia/perfusion up-regulates the expression of IGF-1 in neonatal rats, which may promote the proliferation of neural stem cells. Decrease of the neural stem cells proliferation dramatically after the administration of JB1 suggests that IGF-1 may play a key role on recovery of transient forebrain ischemia damage.

关 键 词：短暂脑缺血 神经发生 胰岛素样生长因子1 免疫组织化学 新生大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]