大鼠实验性胃溃疡自愈期间垂体三叶因子3的变化  

Changes of trefoil factor 3 in hypophysis during healing of experimental gastric ulcer in rats

作　　者：张小俊[1] 吕洋[1] 张文静[1] 张静[1] 邵素霞[2] 吴靖芳[1] 

机构地区：[1]河北北方学院组织学胚胎学教研室,河北张家口075000 [2]河北医科大学组织学胚胎学教研室,石家庄050017

出　　处：《解剖学报》2012年第2期262-267,共6页Acta Anatomica Sinica

基　　金：河北省自然科学基金资助项目(C2007000906)

摘　　要：目的通过观察大鼠实验性胃溃疡形成和愈合过程中垂体和血清三叶因子3(TFF3)的改变,探讨垂体TFF3在实验性胃溃疡愈合中的作用。方法以免疫组织化学染色检测溃疡组(42只)、盐水组(21只)和正常组(6只)大鼠垂体TFF3蛋白的表达;RT-PCR检测TFF3mRNA的转录;酶联免疫吸附测定(ELISA)法检测血清TFF3含量变化,原位杂交技术显示TFF3mRNA的定位。结果大鼠实验性胃溃疡自愈期间TFF3免疫反应阳性物质存在于腺垂体部分细胞和神经垂体,而TFF3 mRNA仅存在于腺垂体。与对照组相比,溃疡第1～6天,腺垂体内阳性信号平均吸光度值明显增大,6d达高峰,10d略有下降,14d复增高,23d下降,但仍高于对照组(P<0.01或P<0.05);神经垂体TFF3平均吸光度值在2～23d高水平波动,14d达高峰(P<0.01或P<0.05)。RT-PCR显示,各组垂体均检测到TFF3 mRNA转录,溃疡组TFF3/GAPDH吸光度比值在溃疡2～23d均高于对照组(P<0.01或P<0.05);血清TFF3含量在溃疡组明显高于正常组(P<0.01)。结论垂体TFF3在溃疡期间高表达,可能通过释放入血发挥促进溃疡愈合的作用。Objective To explore the function of trefoil factor 3 （TFF3） during the self-healing of experimental gastric ulcer in rats,and to observe the changes of TFF3 in hypophysis and blood serum. Methods The expression and changes of TFF3 peptide and mRNA in hypophysis at 42 gastric ulcer, 21 saline and 6 normal rats were detected by immunohistochemical SP and RT-PCR methods respectively. The content of TFF3 peptide of blood serum in different phase was detected by ELISA. In situ hibridazation method （ISH） was used to detect the location of TFF3 mRNA in hypophysis. Results TFF3 immunoreactive profiles were mainly located in some cells of adenohypophysis（AP） , and neurohypophysis （NP） ,but TFF3 mRNA only in some cells of AP by ISH. The average absorbance（AA） values increased from the day 1 to 6 after gastric ulcer,reached its penk on the 6th day,then a little decreased on the day 10, increased on day 14 again,then decreased on the day 23, but remained at a high level compared with the control group（ P 〈 0. O1 or P 〈 0. 05 ）. In NP, there was a high level undulation from day 2 to 23 with the highest on the 14th day （P 〈 0. 01 or P 〈 0.05）. TFF3mRNA transcription was detected in every group. The AA of TFF3/GAPDH was obviously higher in gastric ulcer group on the day 2 to 23 compared with the control group（P 〈0.01 or P 〈0.05）. The content of TFF3 in blood serum was higher than that of the control group（P 〈 0. 01 ）. Conclusions The TFF3 peptide /mRNA are highly expressed in hypophysis during the healing of experimental gastric ulcer in rats,and maybe released into blood serum to play a promotion on gastric ulcer.

关 键 词：三叶因子3 垂体 实验性胃溃疡 反转录-聚合酶链式反应 免疫组织化学 大鼠 

分 类 号：R322.4[医药卫生—人体解剖和组织胚胎学]