毛尖紫萼藓GpAPX的克隆和信息学分析及其在植株干旱与复水下的表达分析  

Cloning,bioinformatics analysis and expression analysis of GpAPX during dehydration and rehydration in Grimmia pilifera

作　　者：宋晓宏[1] 沙伟[2] 金忠民[2] 张艳馥[2] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006

出　　处：《南京农业大学学报》2012年第2期51-58,共8页Journal of Nanjing Agricultural University

基　　金：黑龙江省科技厅农业攻关项目(GC06J101);黑龙江省教育厅海外学人合作项目(1151hz022)

摘　　要：以毛尖紫萼藓(Grimmia pilifera)为试材,采用RACE技术克隆获得1个抗坏血酸过氧化物酶基因(APX)的cDNA序列,命名为GpAPX(GenBank登录号:GU989311)。该基因全长1 115 bp,编码区为771 bp,编码256个氨基酸,5'非翻译区为116 bp,3'非翻译区为228 bp。序列分析表明:该基因属于过氧化物酶基因家族,与其他植物抗坏血酸过氧化物酶的同源性为70.2%～89.7%。实时定量PCR检测结果显示,GpAPX在干旱及复水条件下均能表达,表明GpAPX蛋白可能与抗旱性相关,在毛尖紫萼藓响应干旱胁迫过程中发挥作用。In this study,a full-length cDNA sequence of a ascorbate peroxidase（APX）gene named GpAPX was cloned from Grimmia pilifera（GenBank accession No.GU989311）using rapid amplification of cDNA ends（RACE）.The full-length cDNA of GpAPX was 1 115 bp with a 771 bp open reading frame（ORF）which encoded 256 amino acids and containing a 116 bp 5′UTR and a 228 bp 3′UTR.Sequence analysis showed that GpAPX belonged to the peroxidase protein families.GpAPX protein sharing 70.2%-89.7% homology with APX proteins from other plants.QRT-PCR analysis suggested that the expression of GpAPX gene was induced in both dehydration and rehydration.The results demonstrated that the GpAPX protein may be involved in drought resisitance,and play an important role in Grimmia pilifera through responses to drought stress.

关 键 词：毛尖紫萼藓 干旱胁迫 GpAPX 序列分析 基因表达 

分 类 号：Q949.352[生物学—植物学]