内皮素A受体在肉鸡肺动脉平滑肌细胞增殖中的作用  

Role of endothelin A receptor on the proliferation of broiler pulmonary artery smooth muscle cells

作　　者：柴娟[1] 胡玉霞 毕师诚[1] 谭勋[1] 

机构地区：[1]浙江大学动物科学学院,浙江杭州310058 [2]浙江汇能动物药品有限公司,浙江海宁314422

出　　处：《南京农业大学学报》2012年第2期131-134,共4页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30972221);中央高校基本科研业务费专项资金项目

摘　　要：为探索内皮素-1(ET-1)对肉鸡肺动脉平滑肌细胞(PASMC)体外增殖的影响及其发挥效应的受体途径,采用四甲基偶氮唑蓝(MTT)比色法观察ET-1对PASMC体外增殖的刺激作用,并分别观察内皮素A受体(ETAR)拮抗剂BQ123和B受体(ETBR)拮抗剂BQ788对ET-1作用下的肉鸡PASMC增殖的影响。结果表明:ET-1可显著刺激肉鸡PASMC的增殖(P<0.05),此效应可被BQ123阻断;BQ788对ET-1刺激的PASMC增殖无显著影响(P>0.05)。结论:ET-1具有促肉鸡PASMC增殖的作用,此作用受ETAR介导。This study was designed to investigate the effect of endothelin-1 （ ET-1 ） on the proliferation of broiler pulmonary artery smooth muscle cells（PASMC）and the corresponding receptor involved in this process. The proliferative effect of ET-1 on cultured PASMC was detected with methyl thiazolyl tetrazolium（MTT） assay, and the underlying receptor pathway was evaluated by block of endothelin A receptor（ ETA R） sites us!ng BQ123 and ETBR sites using BQ788,respectively. It was found that the ET-1 significantly enhanced the proliferation of broiler PAMSC （P〈0.05） ;however, this effect was blocked by BQ123. In contrast, BQ788 had no effect on PASMC proliferation stimulated by ET-1 （P〉0.05）. The results demonstrated that ET-1 enhanced the proliferation of broiler PASMC,which was mediated by ETAR rather than ETBR.

关 键 词：内皮素受体 肺动脉平滑肌细胞 肉鸡 

分 类 号：S852.3[农业科学—基础兽医学]