小麦avenin-like type-B基因启动子的分离及表达载体的构建  

Isolation of Avenin-like type-B gene promoter from wheat and construction of expression vector

作　　者：宋斐[1] 陈泠[1,2] 孙杨柳[1] 王菲菲[1] 汪越胜[1] 杨广笑[1] 何光源[1] 

机构地区：[1]华中科技大学生命科学与技术学院中英HUST-Rres基因工程和基因组学联合实验室/科技部国际科技合作基地(基因工程)/教育部分子生物物理重点实验室,湖北武汉430074 [2]湖北省农科院粮食作物研究所,湖北武汉430064

出　　处：《广东农业科学》2012年第4期1-4,共4页Guangdong Agricultural Sciences

基　　金：国家转基因重大专项(2011ZX08010004-004)

摘　　要：根据已知的小麦ALP type-B基因序列,采用反向PCR技术,从小麦品种鄂恩1号(En1)中克隆得到了ALP type-B基因的上游启动子序列1664 bp。通过PLACE和plantCARE等数据库对所克隆的启动子进行生物信息学分析,发现该启动子除具备启动子的基本元件如TATA-box、CAAT-box等外,还含有胚乳特异性表达的特有元件如Endosperm motif、GCN4-like motif(GLM)、RY motif和G-box等。用ALP type-B启动子置换质粒pBI121上的35S启动子,将其与gus基因连接构建植物表达载体。The study successfully cloned a 1 664 bp upsteam sequence of ALP from wheat Enl using Inverse-PCR （IPCR）,based on the sequence of ALP type-B gene.Putative fuctional promoter elements were analyzed by the PLACE datebase and PlantCARE datebase. The results showed that the upsteam sequence contained the basic elements such as CAAT-box and TATA-box. Endosperm motif, GCN4- like motif （GLM）,RY motif and G-box were also detected as endosperm-specific elements.Expression vector harboring the promoter fused with gus gene was constructed by replacing the 35S promoter in pBI121.

关 键 词：小麦 胚乳特异性基因 启动子 植物表达载体 

分 类 号：Q755[生物学—分子生物学] S512.1[农业科学—作物学]