对磺基苯偶氮变色酸褪色光度法测定壳聚糖的研究  

Fading Spectrophotometric Determination of Chitosan with p-sulfophenylaxochomotropic Acid

作　　者：马卫兴[1] 章健[1] 蔡亚雯 马晓冬[1] 

机构地区：[1]淮海工学院化学工程学院,江苏连云港222005

出　　处：《淮海工学院学报（自然科学版）》2012年第1期43-46,共4页Journal of Huaihai Institute of Technology:Natural Sciences Edition

基　　金：2010年江苏省高等学校大学生实践创新训练计划项目(1130);淮海工学院江苏省海洋生物技术重点建设实验室开放课题(2005HS010)

摘　　要：在pH=3.6的Clark-Lubs缓冲溶液缓冲介质中基于对磺基苯偶氮变色酸与壳聚糖发生相互作用,使体系在510nm处褪色,建立测定壳聚糖的新方法。通过实验对该结合反应的条件进行优化,壳聚糖质量浓度在1～12mg/L内服从比尔定律,其工作曲线的回归方程为A=0.017 00ρ+0.031 7,相关系数R为0.999 0。所拟方法用于胶囊中壳聚糖的测定,结果与溴甲酚绿法所得结果一致。It was found that chitosan can react with p-sulfophenylaxochomotropic acid forming a complex in the Clark-Luhs medium at pH 3.6 and room temperature. The fading reaction causes the change of absorption spectra at 510 nm. The optimum conditions of the chitosan were investi-gated. Beer＇s law was obeyed in the concentration range of 1-12 mg chitosan per liter with the regressive equation of A=0. 017 00p（mg/L）-90. 031 7 as well as the coefficient R of 0. 999 0. The proposed method was applied to the determination of chitosan in capsules with satisfactory results in agreement with that of bromocreso green method.

关 键 词：壳聚糖 对磺基苯偶氮变色酸 褪色光度法 

分 类 号：R284.1[医药卫生—中药学]