套式PCR法在肺炎支原体感染诊断中的应用  

Application of Nested PCR on Identification of Mycoplasma Pneumoniae Infection

作　　者：马少杰[1] 辛德莉[2] 

机构地区：[1]民航总医院儿科,北京100123 [2]首都医科大学附属北京友谊医院儿科,北京100050

出　　处：《中国医药指南》2012年第7期1-1,7,共2页Guide of China Medicine

基　　金：北京自然科学基金资助项目(7063075)

摘　　要：目的比较套式PCR法(nested polymerase chain reaction,nPCR)与培养法在早期诊断MP(Mycoplasma pneumoniae,MP)感染敏感性差异。方法选取2007年至2009年间MPP(Mycoplasma pneumoniae pneumonia,MPP)患儿,分别行血清MP-IgM测定、取咽拭子标本行MP分离、鉴定及培养,提取MP-DNA样本,应用nPCR法进行分子鉴定。结果累计选取研究对象65例,nPCR法检测阳性率分别为:16SrRNA-nPCR为75.4%(49例)和23SrRNA-nPCR阳性率为78.5%(51例),无统计学差异(P=0.687);分离培养得到MP31株,培养法MP阳性率47.6%,与nPCR法相比有显著差异(P<0.001)。结论早期检测MP感染nPCR方法比培养法敏感性高。To compare the sensitivity difference between the nested PCR and liquid culture methods in earlydetecting Mycoplasma penmoniae. Methods We chose MP pneumonia children patients from the year of 2007 to 2009. The patients were collected throat swab specimens respectively. DNA samples were extracted to carry on MP specific 16SrRNA and 23SrRNA nested PCR detections; Mp isolatations were done using liquid culture method. Results The positive isolation ratio by nested 16SrRNA PCR is 75.4%（49）, 23SrRNA PCR is78.5 %（51）; there is no stastic difference between the two methods（P=0.687）. 31 MP trains were isolated from 65 samples. Conclusions nPCR was more sensitive than liquid cuituration in early detecting MP infection.

关 键 词：肺炎支原体 套式PCR 

分 类 号：R375.2[医药卫生—病原生物学]