HMGA1在由胶质母细胞瘤细胞株U251分得的胶质瘤干细胞中的表达  

HMGA1 expression in glioma stem cells isolated from the glioblastoma multiforme cell line U251

作　　者：郭华[1] 范海涛[1] 刘滨[1] 贾践博[1] 许尚臣[1] 庞琦[1] 栾立明[1] 

机构地区：[1]山东大学附属省立医院神经外科,济南250021

出　　处：《山东大学学报（医学版）》2012年第3期40-43,49,共5页Journal of Shandong University:Health Sciences

基　　金：山东省卫生厅医药卫生科技发展计划项目(2007HZ064);山东省自然科学基金资助项目(2009HZ053);山东省自然科学基金资助项目(ZR2010HM013);山东省卫生厅科技发展计划项目(2007GG30002010)

摘　　要：目的分析人类胶质母细胞瘤(glioblastoma multiforme,GBM)细胞株U251和由此分离的胶质瘤干细胞中HMGA1的差异性表达。方法用MACS柱从U251中分离出表达表面标志物CD133的胶质瘤干细胞(glioblas-toma stem cells,GSCs),并用免疫荧光技术和流式细胞分析法对其进行分析。用实时反转录酶-聚合酶链反应技术(real-time PCR,RT-PCR)和蛋白质印迹技术分别探测到两类细胞的HMGA1基因在转录和翻译水平上表达的不同。结果从U251中分离出GSCs,U251中的GSCs约为0.32%。在GSCs中,HMGA1在转录和翻译水平上分别为U251蛋白的(6.13±0.25)倍和(2.75±0.99)倍。结论相比于U251,HMGA1在GSCs是过度表达的。HMGA1的过度表达可能与体内肿瘤干细胞的恶性扩增、侵袭和分化密切相关。HMGA1基因可望成为胶质母细胞瘤的一种生物标记物和治疗的靶向目标。Objective To examine HMGA1 expression in glioblastoma cell line U251 and glioma stem cells（GSCs） isolated from it.Methods GSCs expressing the surface marker CD133 were isolated from U251 using an MACS column.Immunofluorescence and flow cytometry analyses were performed on GSCs.GSCs was compared with U251 in HMGA1 expression at the transcriptional（real-time PCR） and translational（Western blotting） levels.Results GSCs were isolated from U251.The percentage of GSCs in U251 was about 0.32%.In GSCs,HMGA1 expression was 6.13±0.25 times higher at the transcriptional level and 2.75±0.99 times higher at the translational level than in U251.Conclusion HMGA1 is overexpressed in GSCs as compared to U251,which implied that HMGA1 plays an important role in malignant proliferation,invasion,and differentiation of tumors in vivo.HMGA1 could be used as a biomarker and a therapeutic target for glioblastoma and GSC.

关 键 词：人类胶质母细胞瘤 胶质瘤干细胞 高迁移率族蛋白A1 

分 类 号：R651.1[医药卫生—外科学]